Detailed information of TA system TA152615

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5531302..5531897
• Replicon: chromosome
• Accession: NZ_CP050335
• Organism: Pseudomonas aeruginosa strain DVT401

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: HB725_RS25795
• Protein ID: WP_003117425.1
• Coordinates: 5531619..5531897 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HB725_RS25790
• Protein ID: WP_003113527.1
• Coordinates: 5531302..5531607 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB725_RS25770	5526786..5527121	+	336	WP_031633682.1	hypothetical protein	-
HB725_RS25775	5527653..5529419	-	1767	WP_023087829.1	hypothetical protein	-
HB725_RS25780	5529597..5530338	-	742	Protein_5093	restriction endonuclease	-
HB725_RS25785	5530562..5530975	-	414	WP_049231965.1	hypothetical protein	-
HB725_RS25790	5531302..5531607	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
HB725_RS25795	5531619..5531897	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HB725_RS25800	5532226..5534454	+	2229	WP_003113525.1	TonB-dependent receptor	-
HB725_RS25805	5534524..5535171	-	648	WP_003095021.1	carbonate dehydratase	-
HB725_RS25810	5535233..5536471	-	1239	WP_003111578.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T152615 WP_003117425.1 NZ_CP050335:c5531897-5531619 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T152615 NZ_CP066358:1360723-1361166 [Sinorhizobium meliloti]
GTGACTTTCCTGCTCGACGTCAATGTGCTGATTGCCTTGATCGATCCGAGCCATATAGGCCATGATGATGCGCACGAATG
GTTCGCGTCGATGGGTCAGACCGCGTGGGCCACCTGCCCAATTACCGAAAATGGTGTCATCCGGATCGTCGGCAATCCCG
AATATCCCAATTCTCCCGGCTCACCGTTACCCGTGATGGAGATCGTCGCCAAATTGCGGTCGCTCCCCGGTCATGTTTTT
TGGCCGGACGATGTGAGCCTTGTCGGGTCAAGTGATATCATCCCATCGAAAATCCTGACTTCGGGGCAGGTGACTGACAC
CTATCTGCTTGCGCTCGCCAAGGCGCGTGGGGGCAAGCTGGCGACATTCGATCGCAAGCTGTCGGCAGCGGCGGTCACGA
AGGGCAATTCCGCCTTGCACCTGATCGCGACGAATAGGTCGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT152615 WP_003113527.1 NZ_CP050335:c5531607-5531302 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT152615 NZ_CP066358:1360499-1360726 [Sinorhizobium meliloti]
ATGAGAACGACCTTGGCTATTGACGACGATGTCCTGATCGCCGCAAAGGCAATGGCGACCCAACAGCAGCGGAGCGTCGG
CGAGGTAATTTCCGAACTGGCGAGGCGTTCTCTGCGCCGGCCGCGCAGCGGCGGCGAGCGTAATGGAATTCCTCTCCTGT
CGCCTCGTCCGGACGCACCGCCCGTGACGCTCGAGATCGTCAACGCCCTGCGTGACGAACTGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5