Detailed information of TA system TA152607

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5192636..5193231
• Replicon: chromosome
• Accession: NZ_CP050334
• Organism: Pseudomonas aeruginosa strain DVT410

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: HB727_RS23875
• Protein ID: WP_003113526.1
• Coordinates: 5192953..5193231 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HB727_RS23870
• Protein ID: WP_003099268.1
• Coordinates: 5192636..5192941 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB727_RS23835	5187787..5188635	+	849	WP_003146058.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
HB727_RS23845	5188802..5189743	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
HB727_RS23850	5189860..5190474	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
HB727_RS23855	5190516..5191100	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
HB727_RS23860	5191141..5192241	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
HB727_RS23870	5192636..5192941	-	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
HB727_RS23875	5192953..5193231	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HB727_RS23880	5193560..5195788	+	2229	WP_023108789.1	TonB-dependent receptor	-
HB727_RS23885	5195858..5196505	-	648	WP_003095021.1	carbonate dehydratase	-
HB727_RS23890	5196567..5197805	-	1239	WP_023108790.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T152607 WP_003113526.1 NZ_CP050334:c5193231-5192953 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T152607 NZ_CP066352:309722-310018 [Bradyrhizobium japonicum]
ATGGCCAGAACCCGACCATTTGATGCAGCAGAGTATCTGGATAGTCCAGAAGCAATCGCAGCCTATCTCAGCGAAGCATT
CGAAACCAATGACCCCGCGTTTATCACCGATGCCATTGGCACCGTTGCTCGCGCTCGCGGAATGACTGCCGTTGCTAAAG
ATGCAGGCCTCTCCCGCGAGCACCTTTACAAAGCACTTAGCTCCGAGGGCCGCCCAGAATTTGGCACCGTTCTCAAGGTG
CTTGAATCTTTAGGGATACATCTACACGCCGAGCCAAGAGTAGCCAAAGTCGCCTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT152607 WP_003099268.1 NZ_CP050334:c5192941-5192636 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT152607 NZ_CP066352:309422-309718 [Bradyrhizobium japonicum]
ATGCCGGCAATAAGAGAAACGGAGCAATTCTCCAGTTGGCTCAGTGACTTACGTGATGTTAGGGCGAAGGCCAAGGTCTT
GGTTAGGGTCGAGAGACTGGCCAAGGGCAACGCGGGAGACGTCAAGCCGGTCGGAGAAGGAATAAGCGAACTTCGCATCC
ACTATGGGGCGGGATACCGAGTTTATTTCACCCAACGCGGTGAAGAGTTGATCCTGCTTCTTTGCGGTGGCGATAAGGAT
TCACAAGACAAGGACATCGCCAACGCAAAGAAGATCGCGAGTGAGTTGGAGGAGTGA

Structures

Toxin

• AlphaFold DB: V6ALY3