Detailed information of TA system TA152602

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5366026..5366621
• Replicon: chromosome
• Accession: NZ_CP050333
• Organism: Pseudomonas aeruginosa strain DVT412

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: HB729_RS24850
• Protein ID: WP_003117425.1
• Coordinates: 5366343..5366621 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HB729_RS24845
• Protein ID: WP_003099268.1
• Coordinates: 5366026..5366331 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB729_RS24810	5361166..5362014	+	849	WP_023103532.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
HB729_RS24820	5362181..5363122	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
HB729_RS24825	5363239..5363853	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
HB729_RS24830	5363895..5364479	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
HB729_RS24835	5364520..5365620	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
HB729_RS24845	5366026..5366331	-	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
HB729_RS24850	5366343..5366621	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HB729_RS24855	5366950..5369178	+	2229	WP_003113525.1	TonB-dependent receptor	-
HB729_RS24860	5369248..5369895	-	648	WP_003095021.1	carbonate dehydratase	-
HB729_RS24865	5369957..5371195	-	1239	WP_003111578.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T152602 WP_003117425.1 NZ_CP050333:c5366621-5366343 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T152602 NZ_CP066344:c7065-6733 [Xanthomonas citri pv. durantae]
ATGGATCGCGGCGATGTCTATTTGGTTTCGCTCGATCCCACGTCCGGCCATGAACAACAGGGCACGCGCCCGGTGCTGAT
TGTGTCGCCAAGCGCGTTCAACCGCCTGACGAAAACACCGGTCGTGCTGCCGATCACCAGTGGGGGTAACTTCGCCCGCA
CGGCAGGCTTTACCGTGTCGCTCATGGGTGCGGGTACCAACACAACTGGCGTAGTTCGCTGCGATCAGCCCCGCGCTCTC
GATCTAGCCTCGCGGCGCGCGCGCAAGCTGGAAACCGTGCCGTCCCCGATCATGGATGAGGTATTAGCGAAGCTGAGCGC
GATCCTTGAGTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT152602 WP_003099268.1 NZ_CP050333:c5366331-5366026 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT152602 NZ_CP066344:c7322-7065 [Xanthomonas citri pv. durantae]
ATGCATACGACCAATCTGCGTAAGGTCGGTGGCTCGATCATGTTGGCTGTCCCGCCCGCCATTCTGGATATGCTGCGTCT
GCGTGCCGGCGCTACCGTTGGCTTGGCCGTCGATCATGGTCGCCTGGTGATCGAACCCACGCTGCGTCCGTACTACAGCC
TGGATGAGCTGCTGGCCCAATGCGACGCATCCGCCGAACTCTCCACCGAAGATCGGGAATGGCTCGACGCCAAGCCTGTT
GGCAGCGAGCTGCTGTGA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5