Detailed information of TA system TA152593

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 5156091..5156772
• Replicon: chromosome
• Accession: NZ_CP050332
• Organism: Pseudomonas aeruginosa strain DVT413

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6AKP0
• Locus tag: HB730_RS24390
• Protein ID: WP_003111825.1
• Coordinates: 5156407..5156772 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HB730_RS24385
• Protein ID: WP_003145733.1
• Coordinates: 5156091..5156414 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB730_RS24360	5152004..5152642	+	639	WP_003163192.1	hypothetical protein	-
HB730_RS24365	5152885..5153220	+	336	WP_003140884.1	TM2 domain-containing protein	-
HB730_RS24370	5153394..5153912	+	519	WP_003140886.1	PAAR domain-containing protein	-
HB730_RS24375	5153909..5154610	+	702	WP_003111822.1	VRR-NUC domain-containing protein	-
HB730_RS24380	5154627..5155715	+	1089	WP_058176146.1	DUF3396 domain-containing protein	-
HB730_RS24385	5156091..5156414	-	324	WP_003145733.1	XRE family transcriptional regulator	Antitoxin
HB730_RS24390	5156407..5156772	-	366	WP_003111825.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HB730_RS24395	5157036..5157275	-	240	WP_044060930.1	hypothetical protein	-
HB730_RS24400	5157482..5157754	+	273	WP_033954259.1	hypothetical protein	-
HB730_RS24405	5157785..5158210	-	426	WP_003116492.1	VOC family protein	-
HB730_RS24410	5158311..5159195	+	885	WP_033939105.1	LysR family transcriptional regulator	-
HB730_RS24415	5159168..5160121	-	954	WP_003085661.1	LysR family transcriptional regulator	-
HB730_RS24420	5160342..5160776	+	435	WP_003114204.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	5143021..5176545	33524

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13880.24 Da
• Isoelectric Point: 4.8219

>T152593 WP_003111825.1 NZ_CP050332:c5156772-5156407 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRHGHMRELRTQHGGRPFRTLYAFDPRRSA
ILLIGGDKTGDDRWYELNVPIADRLYDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T152593 NZ_CP066337:2654842-2655192 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11896.73 Da
• Isoelectric Point: 6.4751

>AT152593 WP_003145733.1 NZ_CP050332:c5156414-5156091 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQARGLSQKMLSEVLHVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT152593 NZ_CP066337:2654555-2654836 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACGACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: V6AKP0