Detailed information of TA system TA152578

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5363316..5363911
• Replicon: chromosome
• Accession: NZ_CP050330
• Organism: Pseudomonas aeruginosa strain DVT779

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HB733_RS24950
• Protein ID: WP_071538299.1
• Coordinates: 5363633..5363911 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HB733_RS24945
• Protein ID: WP_003123432.1
• Coordinates: 5363316..5363621 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB733_RS24925	5358522..5358794	-	273	WP_004352675.1	hypothetical protein	-
HB733_RS24930	5359241..5359576	+	336	WP_025982303.1	hypothetical protein	-
HB733_RS24935	5359636..5361921	-	2286	WP_023131599.1	DEAD/DEAH box helicase	-
HB733_RS24940	5361918..5362649	-	732	WP_023131600.1	hypothetical protein	-
HB733_RS24945	5363316..5363621	-	306	WP_003123432.1	HigA family addiction module antidote protein	Antitoxin
HB733_RS24950	5363633..5363911	-	279	WP_071538299.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HB733_RS24955	5364240..5366468	+	2229	WP_003113525.1	TonB-dependent receptor	-
HB733_RS24960	5366538..5367185	-	648	WP_003095021.1	carbonate dehydratase	-
HB733_RS24965	5367247..5368485	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10616.17 Da
• Isoelectric Point: 7.8937

>T152578 WP_071538299.1 NZ_CP050330:c5363911-5363633 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHGIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T152578 NZ_CP066310:2870668-2870949 [Azotobacter chroococcum]
ATGACCTATAGCCTGGAGTTCCTGCCGTCCGCGCTGAAGGAATGGGAGAAGCTGGGGCACACGGTGCGCGAGCAGCTGAA
GAAGAAACTGCGCGAGCGTCTGGAGCTGCCCAAGGTGCCGGCCGACGCACTGCGCGAGATGCCGAACCACTACAAGATCA
AGCTACGCGCCTCGGGCTATCGGTTGGTGTACCGGGTGGAAGACGAGCGCGTGGTGGTGGTGGTGGTCGCCGTGGGCAAG
CGCGAGCGCAGCGCGGCCTATGAGAAGGCGAAGGGGCGGTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11204.78 Da
• Isoelectric Point: 4.7769

>AT152578 WP_003123432.1 NZ_CP050330:c5363621-5363316 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAQG

Nucleotide

• Length: 306 bp

>AT152578 NZ_CP066310:2870433-2870678 [Azotobacter chroococcum]
ATGCAAAACGTACTGGCCAACAAGGCCGTCAGCGTGTCCGAGCTGAAGAAGAACCCGTCTGCGGTGATCGGCAGCGCCAA
TGGCGGGCCGGTGGCGGTGCTGAATCACAACCGGGTGATGGGTTACATGGTGCCGGCCGAAGCGTTCGAGGCAATGGTCG
AGCGGCTGGAAGACCTGGAGCTGGCCGAACTCGCCCGCTCTCGCGCCGGCGAAACACCCGTGCCGGTGAATCTGGATGAC
CTATAG