Detailed information of TA system TA152577

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4737444..4738125
• Replicon: chromosome
• Accession: NZ_CP050330
• Organism: Pseudomonas aeruginosa strain DVT779

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HB733_RS22080
• Protein ID: WP_015503432.1
• Coordinates: 4737760..4738125 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HB733_RS22075
• Protein ID: WP_003145733.1
• Coordinates: 4737444..4737767 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB733_RS22050	4733357..4733995	+	639	WP_003163192.1	hypothetical protein	-
HB733_RS22055	4734238..4734573	+	336	WP_003140884.1	TM2 domain-containing protein	-
HB733_RS22060	4734747..4735265	+	519	WP_031629200.1	PAAR domain-containing protein	-
HB733_RS22065	4735262..4735963	+	702	WP_023110332.1	VRR-NUC domain-containing protein	-
HB733_RS22070	4735980..4737068	+	1089	WP_003145732.1	DUF3396 domain-containing protein	-
HB733_RS22075	4737444..4737767	-	324	WP_003145733.1	XRE family transcriptional regulator	Antitoxin
HB733_RS22080	4737760..4738125	-	366	WP_015503432.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HB733_RS22085	4738389..4738628	-	240	WP_044060930.1	hypothetical protein	-
HB733_RS22090	4738835..4739107	+	273	WP_023104842.1	hypothetical protein	-
HB733_RS22095	4739138..4739563	-	426	WP_003085665.1	ring-cleaving dioxygenase	-
HB733_RS22100	4739664..4740548	+	885	WP_003085663.1	LysR family transcriptional regulator	-
HB733_RS22105	4740521..4741474	-	954	WP_003085661.1	LysR family transcriptional regulator	-
HB733_RS22110	4741695..4742129	+	435	WP_003158601.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13798.14 Da
• Isoelectric Point: 4.8219

>T152577 WP_015503432.1 NZ_CP050330:c4738125-4737760 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRPGHMRELRTQHGGRPFRTLYAFDPRRSA
ILPIGGDKTGDDRWYELNVPIADRLHDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T152577 NZ_CP066310:c2122644-2122207 [Azotobacter chroococcum]
GTGATCCTCTGGCGGATCAGTGCCTACGCCGATCTCGGCGGAATCGGCGGCCTGCGCGCCAGTGGCCGGTGGCACCACCT
TGGCAGGCCGGTCGTTTACGCGGCGGACAGCCCGGCCGGTGCGATGCTGGAGGTGCTGGTCCATCTGGAAATCGACCCCG
AGGACTTCCCCACGACGCTCCAGTTGATCCGCATCGAGCTTCCAGACCCGGTTTCGCTGGCCGAGCTTCCGCCGCTACCG
GAGCATTGGAAGTCCGACCAGGAGGTCACACGCGCCATGGGTGACCATTTCCTGGATGGGCGGAGCGCGCTACTGCTCCC
GGTGCCAAGCGCAATCATCCCTTGCACGACCAACTACTTGTTCAACCCGGCGCATCCCGAGGCCAACGGGGCGAGCATTC
AGGTAGAGAAGTTTCCCCTCGATCCCCGTCTCCTCTGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11896.73 Da
• Isoelectric Point: 6.4751

>AT152577 WP_003145733.1 NZ_CP050330:c4737767-4737444 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQARGLSQKMLSEVLHVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT152577 NZ_CP066310:c2123090-2122641 [Azotobacter chroococcum]
ATGCTTGCTGAAGTCCTGCGTGACCATGGCTACACCGCTTACCGGGCGCGTCTTCAAAATCTGCTGGATATCCCGGAGTC
GGCAACCGACTTTGAAATCCACACCCGGATCACCAAGGGCTTCCCTGCTGCACGGCTGATGCAGCTGACCGAGGCCGGGG
TCGTCACGCCGCTGGAGCGCGACCAGATCATTCCCCTGCGCACCCTCAAGACTCGCATCGAGCGCGATCAGCCGTTGAGC
GTGGAAGAAAGCGACAGGTTTTTTCGCGCCGCTCACATCACGGCAATGGCCGAGGCTGTCTTCGGCGACCGCGAGAAGGC
CAGGCGCTGGCTCTCCAAGCCAAAGGAGCGATTTTCCGGCCTGACGCCCATGCAGATGCTCACCACTCAGCAGGGCACCA
GCCAGGTGGAGGAAATGCTGCTGCAAATCATCGAAGGCCTAGCCCTGTGA