Detailed information of TA system TA152572

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5265696..5266291
• Replicon: chromosome
• Accession: NZ_CP050329
• Organism: Pseudomonas aeruginosa strain DVT417

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: HB734_RS24420
• Protein ID: WP_003117425.1
• Coordinates: 5266013..5266291 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HB734_RS24415
• Protein ID: WP_003099268.1
• Coordinates: 5265696..5266001 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB734_RS24380	5260848..5261696	+	849	WP_033988710.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
HB734_RS24390	5261863..5262804	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
HB734_RS24395	5262921..5263535	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
HB734_RS24400	5263577..5264161	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
HB734_RS24405	5264202..5265302	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
HB734_RS24415	5265696..5266001	-	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
HB734_RS24420	5266013..5266291	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HB734_RS24425	5266620..5268848	+	2229	WP_023086667.1	TonB-dependent receptor	-
HB734_RS24430	5268918..5269565	-	648	WP_033988711.1	carbonate dehydratase	-
HB734_RS24435	5269627..5270865	-	1239	WP_033988712.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T152572 WP_003117425.1 NZ_CP050329:c5266291-5266013 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T152572 NZ_CP066310:c68417-68130 [Azotobacter chroococcum]
ATGCACGTCATTACTCAGAAACGCATCTGGGAAGCCAAGGCCAAGTGGCCGCATGCGGCCACTGCCTTGGAGGCCTGGTA
TCGCCTAATCAAGGCCAGTTCTCCACAGGACTTCGCGGAGATGAAGCATCTGTTTCCGGCAACAGACAAGGTGGGGCCGC
AGCATGTGTTCGACATCGGCGGCAACAAGCTTCGCCTGATCGCCGTTGTGCAGTACCGGGCGCAGAAGGTCTATATCCAG
CATGTGCTGGATCATGGCGAATACGACCAAGGTAAGTGGAGGGAATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT152572 WP_003099268.1 NZ_CP050329:c5266001-5265696 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT152572 NZ_CP066310:c68127-67726 [Azotobacter chroococcum]
ATGTCCGCGCTGATCAAACAAGCCAGCGAACACTGGCGCTATGTGGCCCCCTTGCTCACCAGGCCGGCCAACGAAGACGA
CTACGACGCCTTGGTCGAAGCCCTGGACGAATTGCTGGTTATCGTCGGCGACGACGAAGATCATCCCCTTGCCACCCTGG
CCTCGCACATGGGCGACCTGATCGAAGCCTACGATGAAGCCCAGCGTCCTATGCCGAAGGTGAGCGGCGTGGAGATGCTG
CGCTACCTGATGCAGGAGCATGGTCTGACTCAGGCCGATCTGCCGGAGATCGGCGTGCAGTCCGTCGTTTCCGAGCTGCT
CAGCGGCAAGCGCCGGCTCAATGTGCGCCATATCCGCGCGTTATCCGAGCGCTTTGGTGTACCGGCGGATGTTTTCTTTT
GA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5