Detailed information of TA system TA152561

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 732124..732719
• Replicon: chromosome
• Accession: NZ_CP050328
• Organism: Pseudomonas aeruginosa strain DVT419

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: HB736_RS03470
• Protein ID: WP_003113526.1
• Coordinates: 732124..732402 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HB736_RS03475
• Protein ID: WP_003113527.1
• Coordinates: 732414..732719 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB736_RS03455	727550..728788	+	1239	WP_033970863.1	C69 family dipeptidase	-
HB736_RS03460	728850..729497	+	648	WP_003095021.1	carbonate dehydratase	-
HB736_RS03465	729567..731795	-	2229	WP_033942306.1	TonB-dependent receptor	-
HB736_RS03470	732124..732402	+	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HB736_RS03475	732414..732719	+	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
HB736_RS03485	733114..734214	-	1101	WP_033942321.1	redox-regulated ATPase YchF	-
HB736_RS03490	734255..734839	-	585	WP_033942324.1	aminoacyl-tRNA hydrolase	-
HB736_RS03495	734881..735495	-	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
HB736_RS03500	735612..736553	-	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
HB736_RS03510	736720..737568	-	849	WP_003099284.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T152561 WP_003113526.1 NZ_CP050328:732124-732402 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T152561 NZ_CP066294:1717679-1718194 [Streptococcus mutans]
ATGTTTGACCTTGTTGTTAATTTGATTTTATTGGTTATTGTGATAGGTGGATTTGTCTTCTTAAGATTCTATGCGGATAA
GAAAGGCAAGCGAGAATACGATGAACGCCAGTTACTTATGCAAAAGAAAGCTTATACCAATGCGGCTTGGGTTGTTATGG
GTTTCAATCTTGTTCTTGTGATATGGGGTGAAGTGTTAGCAAAATATATCTCGCTATCCTTTGCAGGTACTGCCAATCTC
TTTCTTATCGTAGGCGTCTTTGTCTGCCACAGTATCTTAAACGATGCTTATTTCACAGCAAGAAAAAACAAAAAATTCCT
CTATGTGTATGCCGTTATTATTGCTGTTCAAATTTTCACTGTTTATCAAAATTGGAGTCAAGGCAGCTTTGGTCATGACG
GACATATTTATCTAACAGGAGAAAAAGCAATGAGTTTATTGTTTATCTTGACTTTTGCTGTCATTTTTCTAGTAACTGCT
TATAAAACGATTCAGGATAAGAGAGAGGGAAAGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT152561 WP_003113527.1 NZ_CP050328:732414-732719 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT152561 NZ_CP066294:1718194-1718640 [Streptococcus mutans]
ATGAAAATGGAAAAGTCAAATAAACAAGTCATTTACGATGAACGTCAGCAGCAAATTCAACTTAAAAGTTATAGTCTTTC
ATTTTGGTTTGTTATGTTTATACTCTATTTTGCAACCTTTGGAAAGGCTGATTTGTTGCTTAATATCGCTTTTTGGGGAG
GACTTGTTCTTAATTTTTGCTACAGTACTCTAAGAGGTGTCGGTCCTTTTGTTGACCCACGTTTTGGAAAAATTGCAAAA
ATCGGTCGTTTGGCCGCTGTTCCTCTTATTTTTTTGGGAATGCTAGTCTTTCTTGTTGCTATCATAATGTCTATTCTAGA
ACATGATAGTTTAAGAGAATCTATCACAAAATGCTCCTACTTAGGCTTATCTGGTTTTTGGCTAATTTGTATGGGAGCAA
GTATCATCTACCGTCATTATCTTGATAAGAAAGAAGCAGACAAATGA

Structures

Toxin

• AlphaFold DB: V6ALY3