Detailed information of TA system TA152556

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 724719..725314
• Replicon: chromosome
• Accession: NZ_CP050327
• Organism: Pseudomonas aeruginosa strain DVT421

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HB737_RS03395
• Protein ID: WP_071538299.1
• Coordinates: 724719..724997 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HB737_RS03400
• Protein ID: WP_003123432.1
• Coordinates: 725009..725314 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB737_RS03380	720145..721383	+	1239	WP_003113524.1	dipeptidase	-
HB737_RS03385	721445..722092	+	648	WP_003095021.1	carbonate dehydratase	-
HB737_RS03390	722162..724390	-	2229	WP_003113525.1	TonB-dependent receptor	-
HB737_RS03395	724719..724997	+	279	WP_071538299.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HB737_RS03400	725009..725314	+	306	WP_003123432.1	HigA family addiction module antidote protein	Antitoxin
HB737_RS03410	725721..726821	-	1101	WP_003099270.1	redox-regulated ATPase YchF	-
HB737_RS03415	726862..727446	-	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
HB737_RS03420	727488..728102	-	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
HB737_RS03425	728219..729160	-	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
HB737_RS03435	729327..730175	-	849	WP_003123430.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10616.17 Da
• Isoelectric Point: 7.8937

>T152556 WP_071538299.1 NZ_CP050327:724719-724997 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHGIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T152556 NZ_CP066272:12745-13017 [Pseudomonas tremae]
GTGCTAGTTGAGTGGCTTCCACAAGCTTTGGAGGCACTGGAGGAAATCGCCGGCTACCTAGCCGAGCGCAACCCTTATGC
TGCCGAGCAGATGCAGGCCACCCTTGAGGCCACGGTCGAGGCTCTCCCCCTGCACCCTTACATCCACCGTCCCGGCCGTG
TTCCAGGAACGCGCGAAGCGGTTGTGCATCCCAACTACCTGGTTGTCTACCGCGTCGGTACAGAGCGCATCAGCATCTTG
GACGTCCTGCACGCCCGCCAAGAGTACCCCTAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11204.78 Da
• Isoelectric Point: 4.7769

>AT152556 WP_003123432.1 NZ_CP050327:725009-725314 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAQG

Nucleotide

• Length: 306 bp

>AT152556 NZ_CP066272:12570-12755 [Pseudomonas tremae]
ATGAGCATTCAACTTGACCCTCGCGTCTCGGAGTTCGAGACCCAGGAACAGGCCGACAATTACGACCGTTGGTTTCGCCA
GCGTATCGAGCACAGTTTGGCCGATCCGCGTCCACCCGTGCCGCACGATGAAGCAATGGCTCGCGTCAGAGCCATGATCG
AAGCAAAGCGCCGCCGTGCTAGTTGA