Detailed information of TA system TA152554

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5477076..5477671
• Replicon: chromosome
• Accession: NZ_CP050325
• Organism: Pseudomonas aeruginosa strain DVT425

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HB740_RS25495
• Protein ID: WP_071535843.1
• Coordinates: 5477393..5477671 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0S3KUN4
• Locus tag: HB740_RS25490
• Protein ID: WP_003111575.1
• Coordinates: 5477076..5477381 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB740_RS25455	5472216..5473064	+	849	WP_034013622.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
HB740_RS25465	5473231..5474172	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
HB740_RS25470	5474289..5474903	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
HB740_RS25475	5474945..5475529	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
HB740_RS25480	5475570..5476670	+	1101	WP_034052802.1	redox-regulated ATPase YchF	-
HB740_RS25490	5477076..5477381	-	306	WP_003111575.1	HigA family addiction module antidote protein	Antitoxin
HB740_RS25495	5477393..5477671	-	279	WP_071535843.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HB740_RS25500	5478000..5480228	+	2229	WP_021264080.1	TonB-dependent receptor	-
HB740_RS25505	5480299..5480946	-	648	WP_003095021.1	carbonate dehydratase	-
HB740_RS25510	5481008..5482246	-	1239	WP_034013624.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10674.25 Da
• Isoelectric Point: 7.8937

>T152554 WP_071535843.1 NZ_CP050325:c5477671-5477393 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRVGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T152554 NZ_CP066271:67189-67539 [Pseudomonas tremae]
ATGAATACGTTTGCACTGCGCTTTACCGATGTTGCCCAGCAAAGCCTTGAGGATCAGGTTGAACACCTGGCTGTCCATCA
GGGATTCTCATCAGCTGCTCAACGCATAGATTCCCTTATCGATGCCATCCAGGACAAGCTCCTGTCCACCCCTTTGGGCT
ACCCGGTCAGTCCTCAATTGAGTGAGCTGGGTGTACTGCACTATCGTGAGTTGAACACTGACGGATACCGGATCTTTTAC
GAGGTGATGGATTCAGAAGGTATTACAGTCATCGTCGTATTGCTCGTATTGGGTGGTAAACAGAGTGTTGAACAGGCTTT
GATCCGCTACTGTCTGCTACAGCCTATTTAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11205.72 Da
• Isoelectric Point: 4.4475

>AT152554 WP_003111575.1 NZ_CP050325:c5477381-5477076 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAQG

Nucleotide

• Length: 306 bp

>AT152554 NZ_CP066271:66928-67176 [Pseudomonas tremae]
ATGCGAGTTGAAACAATTAGCTATCTGAAACGCAACGCAGCCGACCTGCCTCTCGATGAGCCATTGGTCGTCACGCAAAA
CGGTGTCCCGGCCTACGTGGTGGAGTCTTACGCTGATCGTAAGCGCCGCGATGAGTCAATCGCCTTGGTGAAGCTCCTGG
CAATCAGCTCCCGCGAGTACGCCCAAGGCAAGCACTGTTCCACTGACGAACTCAAAGCCCGATTGAGCAGGAGGTTTATC
CAGAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3KUN4