Detailed information of TA system TA152550

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4718542..4719223
• Replicon: chromosome
• Accession: NZ_CP050325
• Organism: Pseudomonas aeruginosa strain DVT425

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HB740_RS21905
• Protein ID: WP_174707904.1
• Coordinates: 4718858..4719223 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1C7BMJ2
• Locus tag: HB740_RS21900
• Protein ID: WP_003159602.1
• Coordinates: 4718542..4718865 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB740_RS21865	4713798..4714676	+	879	WP_034012987.1	hypothetical protein	-
HB740_RS21875	4715306..4717015	+	1710	WP_174707903.1	TIGR04141 family sporadically distributed protein	-
HB740_RS21880	4717001..4717537	-	537	WP_073655295.1	tyrosine-type recombinase/integrase	-
HB740_RS21885	4717645..4717920	-	276	WP_004353146.1	hypothetical protein	-
HB740_RS21890	4717920..4718252	-	333	WP_021205399.1	hypothetical protein	-
HB740_RS21895	4718307..4718426	+	120	Protein_4331	IS5/IS1182 family transposase	-
HB740_RS21900	4718542..4718865	-	324	WP_003159602.1	XRE family transcriptional regulator	Antitoxin
HB740_RS21905	4718858..4719223	-	366	WP_174707904.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HB740_RS21910	4719516..4719776	-	261	WP_079386856.1	hypothetical protein	-
HB740_RS21915	4719983..4720255	+	273	WP_033942888.1	hypothetical protein	-
HB740_RS21920	4720286..4720711	-	426	WP_003085665.1	ring-cleaving dioxygenase	-
HB740_RS21925	4720812..4721696	+	885	WP_003085663.1	LysR family transcriptional regulator	-
HB740_RS21930	4721669..4722622	-	954	WP_003085661.1	LysR family transcriptional regulator	-
HB740_RS21935	4722843..4723277	+	435	WP_003116494.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13910.27 Da
• Isoelectric Point: 4.6844

>T152550 WP_174707904.1 NZ_CP050325:c4719223-4718858 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEEWGPSLGHPHSSGINGSRHGHMRELRTQHGGRPFRTLYAFDPRRSA
ILLIGGDKTGDDRWYELNVPIADRLYDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T152550 NZ_CP066265:3717120-3717434 [Pseudomonas syringae]
ATGATCTTCATCGAAACTCCCGTATTCACTTCGGACCTCAGAGATCATTTGGATGATGAAGCATACAGGGCTCTACAAAT
CTATCTGGCTGAGCATCCAGAATCTGGAGCGCTTATCGAAGATACAGGTGGCCTGCGAAAAATCCGCTGGGCGGCTCATG
GCAAGGGGAAAAGCGGGGGCGTGCGAGTTATTTATTACCATGTCTCTGCGCGATTCCAGATACGCATGATCTTGATCTAT
CGCAAAGGAATCCAGGATTCGCTGACGGACAAACAGAAGGCTCAGCTTCGCGCTATCAACAACGGGTGGAAATAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11882.71 Da
• Isoelectric Point: 6.4751

>AT152550 WP_003159602.1 NZ_CP050325:c4718865-4718542 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEGQARVEAKAQELLAEMPLNELRQARGLSQKMLSEVLHVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT152550 NZ_CP066265:3717434-3717760 [Pseudomonas syringae]
ATGGAAAAAAATCTCTTTGATCGTCTTGTAGAAAGCATGACCGAGATGGACGAGATAGATCGCGGTGAGCGCCAGCCCTC
GCGTGAATTCCATATCGATGCCGTTCAAGTCAAAAAAATCAGACAAGCGACAGGTCTTTCCCAGGCAACCTTTGCCAAGC
GCATTGATGTCGCAGTCGGGACACTTCGTAATTGGGAACAGGGACGACGCGAGCCAGAGGGGCCAGCGCGTGCATTGCTT
CGAGCGATACACAATGATCCAGAGCACGTTCTTGCAGCATTGAGTTTTAACAGGGGTGAAATCGCAGACACAAAGCGCCT
GAACTGA

Structures

Antitoxin

• AlphaFold DB: A0A1C7BMJ2