152545

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-VapI
Location 5419435..5420030 Replicon chromosome
Accession NZ_CP050324
Organism Pseudomonas aeruginosa strain DVT427

Toxin (Protein)


Gene name higB Uniprot ID V6ALY3
Locus tag HB742_RS25105 Protein ID WP_003113526.1
Coordinates 5419752..5420030 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HB742_RS25100 Protein ID WP_003113527.1
Coordinates 5419435..5419740 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HB742_RS25065 5414575..5415423 + 849 WP_003099284.1 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase -
HB742_RS25075 5415590..5416531 + 942 WP_003099281.1 ribose-phosphate pyrophosphokinase -
HB742_RS25080 5416648..5417262 + 615 WP_003099279.1 50S ribosomal protein L25/general stress protein Ctc -
HB742_RS25085 5417304..5417888 + 585 WP_003099278.1 aminoacyl-tRNA hydrolase -
HB742_RS25090 5417929..5419029 + 1101 WP_003099270.1 redox-regulated ATPase YchF -
HB742_RS25100 5419435..5419740 - 306 WP_003113527.1 HigA family addiction module antidote protein Antitoxin
HB742_RS25105 5419752..5420030 - 279 WP_003113526.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HB742_RS25110 5420359..5422587 + 2229 WP_033876905.1 TonB-dependent receptor -
HB742_RS25115 5422657..5423304 - 648 WP_003095021.1 carbonate dehydratase -
HB742_RS25120 5423366..5424604 - 1239 WP_033876906.1 C69 family dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(22-69)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10646.19 Da        Isoelectric Point: 7.8937

>T152545 WP_003113526.1 NZ_CP050324:c5420030-5419752 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Download         Length: 279 bp

>T152545 NZ_CP066256:148415-148564 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11247.81 Da        Isoelectric Point: 5.0747

>AT152545 WP_003113527.1 NZ_CP050324:c5419740-5419435 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Download         Length: 306 bp

>AT152545 NZ_CP066256:c148370-148311 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6ALY3

Antitoxin

Source ID Structure

References