Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4804924..4805605 Replicon chromosome
Accession NZ_CP050324
Organism Pseudomonas aeruginosa strain DVT427

Toxin (Protein)


Gene name higB Uniprot ID V6AKP0
Locus tag HB742_RS22295 Protein ID WP_003111825.1
Coordinates 4805240..4805605 (-) Length 122 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1C7BMJ2
Locus tag HB742_RS22290 Protein ID WP_003159602.1
Coordinates 4804924..4805247 (-) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HB742_RS22255 4800381..4801259 + 879 WP_003163191.1 hypothetical protein -
HB742_RS22265 4801889..4803598 + 1710 WP_003085674.1 TIGR04141 family sporadically distributed protein -
HB742_RS22270 4803584..4803919 - 336 WP_079276585.1 tyrosine-type recombinase/integrase -
HB742_RS22275 4804027..4804302 - 276 WP_004353146.1 hypothetical protein -
HB742_RS22280 4804302..4804634 - 333 WP_021205399.1 hypothetical protein -
HB742_RS22285 4804689..4804808 + 120 Protein_4409 IS5/IS1182 family transposase -
HB742_RS22290 4804924..4805247 - 324 WP_003159602.1 XRE family transcriptional regulator Antitoxin
HB742_RS22295 4805240..4805605 - 366 WP_003111825.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HB742_RS22300 4805896..4806264 - 369 WP_162961515.1 hypothetical protein -
HB742_RS22305 4806277..4806549 + 273 WP_031805311.1 hypothetical protein -
HB742_RS22310 4806580..4807005 - 426 WP_003140891.1 VOC family protein -
HB742_RS22315 4807106..4807990 + 885 WP_031805310.1 LysR family transcriptional regulator -
HB742_RS22320 4807963..4808916 - 954 WP_003085661.1 LysR family transcriptional regulator -
HB742_RS22325 4809137..4809571 + 435 WP_003116494.1 RidA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-106)

Antitoxin

Cro/C1-type helix-turn-helix domain

(33-63)


Sequences

Toxin        


Download         Length: 122 a.a.        Molecular weight: 13880.24 Da        Isoelectric Point: 4.8219

>T152544 WP_003111825.1 NZ_CP050324:c4805605-4805240 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRHGHMRELRTQHGGRPFRTLYAFDPRRSA
ILLIGGDKTGDDRWYELNVPIADRLYDEHLHQLREEGLIDG

Download         Length: 366 bp

>T152544 NZ_CP066256:c103390-102974 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 108 a.a.        Molecular weight: 11882.71 Da        Isoelectric Point: 6.4751

>AT152544 WP_003159602.1 NZ_CP050324:c4805247-4804924 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEGQARVEAKAQELLAEMPLNELRQARGLSQKMLSEVLHVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Download         Length: 324 bp

>AT152544 NZ_CP066256:c103617-103387 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6AKP0

Antitoxin

Source ID Structure
AlphaFold DB A0A1C7BMJ2

References