Detailed information of TA system TA152532

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5581730..5582325
• Replicon: chromosome
• Accession: NZ_CP050322
• Organism: Pseudomonas aeruginosa strain DVT729

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: HB747_RS25905
• Protein ID: WP_003117425.1
• Coordinates: 5582047..5582325 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HB747_RS25900
• Protein ID: WP_003113527.1
• Coordinates: 5581730..5582035 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB747_RS25865	5576869..5577717	+	849	WP_023114865.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
HB747_RS25875	5577884..5578825	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
HB747_RS25880	5578942..5579556	+	615	WP_003095013.1	50S ribosomal protein L25/general stress protein Ctc	-
HB747_RS25885	5579598..5580182	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
HB747_RS25890	5580223..5581323	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
HB747_RS25900	5581730..5582035	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
HB747_RS25905	5582047..5582325	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HB747_RS25910	5582654..5584882	+	2229	WP_003113525.1	TonB-dependent receptor	-
HB747_RS25915	5584952..5585599	-	648	WP_003095021.1	carbonate dehydratase	-
HB747_RS25920	5585661..5586899	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T152532 WP_003117425.1 NZ_CP050322:c5582325-5582047 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T152532 NZ_CP066254:878268-878675 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT152532 WP_003113527.1 NZ_CP050322:c5582035-5581730 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT152532 NZ_CP066254:878021-878287 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5