Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1873368..1873980 Replicon chromosome
Accession NZ_CP050244
Organism Erwinia amylovora strain TS3238

Toxin (Protein)


Gene name hicA Uniprot ID D2TUP9
Locus tag EHX00_RS08310 Protein ID WP_012906750.1
Coordinates 1873801..1873980 (-) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID D4I356
Locus tag EHX00_RS08305 Protein ID WP_004157632.1
Coordinates 1873368..1873778 (-) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EHX00_RS08275 1869026..1869640 - 615 WP_004166850.1 acetyltransferase -
EHX00_RS08280 1870246..1870518 + 273 WP_004157637.1 hypothetical protein -
EHX00_RS08285 1870529..1870702 + 174 WP_013036045.1 hypothetical protein -
EHX00_RS08290 1870797..1870970 + 174 WP_004157636.1 hypothetical protein -
EHX00_RS08295 1871228..1871440 + 213 WP_004157635.1 hypothetical protein -
EHX00_RS08300 1872311..1873312 - 1002 WP_004157633.1 acyltransferase -
EHX00_RS08305 1873368..1873778 - 411 WP_004157632.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
EHX00_RS08310 1873801..1873980 - 180 WP_012906750.1 type II toxin-antitoxin system HicA family toxin Toxin
EHX00_RS08315 1874500..1875228 - 729 WP_004157629.1 two-component system response regulator RstA -
EHX00_RS08320 1875405..1875869 + 465 WP_004157626.1 hypothetical protein -
EHX00_RS08325 1875873..1877264 - 1392 WP_004157625.1 amino acid permease -
EHX00_RS08330 1877472..1878422 - 951 WP_004157624.1 DUF1471 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1866712..1882826 16114


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-126)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6644.85 Da        Isoelectric Point: 10.9132

>T152414 WP_012906750.1 NZ_CP050244:c1873980-1873801 [Erwinia amylovora]
MDSRNAIAMIEADGWYLVRVKGSHHQFKHPTKKGLVTVKHPQKDIPLPTLKSIKKQAGL

Download         Length: 180 bp

>T152414 NZ_CP066144:4269466-4269618 [Escherichia coli]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 14829.76 Da        Isoelectric Point: 4.3623

>AT152414 WP_004157632.1 NZ_CP050244:c1873778-1873368 [Erwinia amylovora]
MLYPVAIDKGDSSFGVRVPDIPGCFSGGDNYQDAIESAREAIDAHIELLVEDGEAVPEATSVENWLADPDYVGVVWALVD
VDVTRLMGKAEKINVTLPSLLIRRIDQFVAAHPEYGSRSGFLSRVAADKVIGREKR

Download         Length: 411 bp

>AT152414 NZ_CP066144:c4269417-4269360 [Escherichia coli]
GTTCTGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7D6XDK4

Antitoxin

Source ID Structure
AlphaFold DB A0A830ZVY0

References