Detailed information of TA system TA152400

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 580483..580741
• Replicon: chromosome
• Accession: NZ_CP050242
• Organism: Erwinia amylovora strain FB-307

Toxin (Protein)

• Gene name: hok
• Uniprot ID: D4I1C1
• Locus tag: EHW99_RS02520
• Protein ID: WP_004160031.1
• Coordinates: 580583..580741 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	580483..580529 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EHW99_RS02480	575929..576072	-	144	WP_004160049.1	hypothetical protein	-
EHW99_RS02485	576545..576937	-	393	WP_004160048.1	DoxX family protein	-
EHW99_RS02490	577147..577428	-	282	WP_004160039.1	YqjK-like family protein	-
EHW99_RS02495	577428..577820	-	393	WP_004160038.1	phage holin family protein	-
EHW99_RS02500	577822..578127	-	306	WP_004160037.1	DUF883 family protein	-
EHW99_RS02505	578170..578541	-	372	WP_004160036.1	DUF1090 domain-containing protein	-
EHW99_RS02510	578709..579101	-	393	WP_004160035.1	EnvZ/OmpR regulon moderator MzrA	-
EHW99_RS02515	579098..579772	-	675	WP_004160034.1	DedA family protein	-
-	580483..580529	-	47	-	-	Antitoxin
EHW99_RS02520	580583..580741	+	159	WP_004160031.1	type I toxin-antitoxin system Hok family toxin	Toxin
EHW99_RS02525	580885..582117	-	1233	WP_004160030.1	serine/threonine transporter SstT	-
EHW99_RS02530	582352..583332	-	981	WP_013035818.1	TerC family protein	-
EHW99_RS02535	583745..585436	+	1692	WP_004160025.1	chloride channel protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6038.32 Da
• Isoelectric Point: 8.4901

>T152400 WP_004160031.1 NZ_CP050242:580583-580741 [Erwinia amylovora]
MKLPANCLIWCVLIVCVTLLIFTYLTRKSLCEIRYKDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T152400 NZ_CP066144:997807-998214 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin

Nucleotide

• Length: 47 bp

>AT152400 NZ_CP050242:c580529-580483 [Erwinia amylovora]
GCATGCAGATGGCCTCGTGGATTAATGAAAATTAACTACGGGGCTTT

Structures

Toxin

• AlphaFold DB: A0A831A7I1

Antitoxin