Detailed information of TA system TA152373

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3699552..3700246
• Replicon: chromosome
• Accession: NZ_CP050173
• Organism: Escherichia coli strain STB20-1

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: FIP50_RS18030
• Protein ID: WP_001263493.1
• Coordinates: 3699552..3699950 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: FIP50_RS18035
• Protein ID: WP_000554757.1
• Coordinates: 3699953..3700246 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FIP50_RS18000	3695111..3695569	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
FIP50_RS18005	3695830..3697287	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
FIP50_RS18010	3697344..3697865	-	522	Protein_3524	peptide chain release factor H	-
FIP50_RS18015	3697864..3698067	-	204	Protein_3525	RNA ligase RtcB family protein	-
FIP50_RS18020	3698313..3698765	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
FIP50_RS18025	3698839..3699536	+	698	Protein_3527	IS1 family transposase	-
FIP50_RS18030	3699552..3699950	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
FIP50_RS18035	3699953..3700246	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
FIP50_RS18040	3700298..3701353	-	1056	WP_001226164.1	DNA polymerase IV	-
FIP50_RS18045	3701424..3702209	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
FIP50_RS18050	3702181..3703893	+	1713	Protein_3532	flagellar biosynthesis protein FlhA	-
FIP50_RS18055	3704117..3704614	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3612987..3705416	92429
	-	flank	IS/Tn	-	-	3699243..3699536	293

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T152373 WP_001263493.1 NZ_CP050173:c3699950-3699552 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T152373 NZ_CP066137:1738759-1739112 [Morganella morganii]
GTGTGGAAGATAATTACAACAGATAATTTTGCAGCGTGGTTTGCTGCGCAGGATGACGGGGATCGTACAGCAATACTGGC
AGCGATGCTGGTATTGGAGAAGACCGGACCCGGTTTATCAGGACCGTATGCGGATACTGTGAAAGGTTCGCGTTATACGA
ATATGAAAGAGTTACGTATACAAAGCAGAGGGCAGCCGATAAGGGCATTTTTTGCATTCGACCCTGAACGGAGAGGTGTT
TTGCTGTGCGCAGGGTATAAAACCGGCAAAGAAAAACGTTTTTATATGCAGATGATTCCGCAGGCTGACCGTGAGTATAC
AGAATGGCTGAACAGGCCGGAAAACAGGAGTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT152373 WP_000554757.1 NZ_CP050173:c3700246-3699953 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT152373 NZ_CP066137:1739115-1739417 [Morganella morganii]
ATGGGAAAGTCACTCAGACAACTGCTGGCTGAGGAAAAGCCGGAAGTCGCGGCAGCAGCACGGGTTCAGGCGGACGAGAT
CCTGCTGAATATCCATCTGGCGGAACTGCGGGAGAAAGTGAACAAAACACAGGTGGAAATGGCACAGGCGCTGGGGATCA
AGCAGCCGACGGTCGCCGGGATGGAAAAACCGGGGCGTGATGTCAAATTATCCACGCTGAGACGCTATGTGGACGCCATC
GGCGGAAAACTGAATATTGATGTTGAGCTGCCGGACGGCACGCATTATATGTTCAGGGTCTGA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6