Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 629022..629821 Replicon chromosome
Accession NZ_CP050173
Organism Escherichia coli strain STB20-1

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag FIP50_RS03025 Protein ID WP_000347273.1
Coordinates 629022..629486 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag FIP50_RS03030 Protein ID WP_001307405.1
Coordinates 629486..629821 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FIP50_RS02995 624023..624457 - 435 WP_064670337.1 PTS sugar transporter subunit IIA -
FIP50_RS03000 624475..625353 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
FIP50_RS03005 625343..626122 - 780 WP_000406209.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
FIP50_RS03010 626133..626606 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
FIP50_RS03015 626629..627909 - 1281 WP_064670336.1 tagatose-bisphosphate aldolase subunit KbaZ -
FIP50_RS03020 628158..628967 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
FIP50_RS03025 629022..629486 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
FIP50_RS03030 629486..629821 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
FIP50_RS03035 629970..631541 - 1572 WP_001273753.1 galactarate dehydratase -
FIP50_RS03040 631916..633250 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
FIP50_RS03045 633266..634036 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T152356 WP_000347273.1 NZ_CP050173:c629486-629022 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T152356 NZ_CP066129:c483096-482845 [Morganella morganii]
ATGAAAACATTAACATACAGTGAAGTACGGCAGAATCTCACCGCTGCGATGAATGAAACAGTGGATGACCGTATCCCTAT
TTTGATTACCCGGCAGAAAGGCACTGCATGTGTATTATTATCACTTGATGAATATCAGTCTCTGGAAGAGACCGCCTATT
TAATGCGTTCACCTAATAACGCGCGACGTTTAATGGATTCCATTGATGAATTGAATGCCGGCAAGGGACTCAAAAAGGAA
ATCAATGAATGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT152356 WP_001307405.1 NZ_CP050173:c629821-629486 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT152356 NZ_CP066129:c482848-482594 [Morganella morganii]
ATGACATTGACGTTTTCGTCTCAGGCATGGGAAGACTATCTGTACTGGCAAAATACAGATAAAAAAACCCTCAGACGTAT
CAACGAATTAATTAAAGCTGCACAAAGAACGCCTTTCTCAGGTATAGGAAAGCCGGAACCACTGAAGAATAATCTCTCCG
GCTGGTGGTCGCGGCGAATAACAGAAGAACACCGGTTAGTTTACCGTGTCACTAACCAGGGTGTTGAGGTGGCGGCCTGC
CGGTATCATTACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References