Detailed information of TA system TA152310

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5472434..5473029
• Replicon: chromosome
• Accession: NZ_CP050149
• Organism: Pseudomonas aeruginosa strain CHA

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HBA22_RS25570
• Protein ID: WP_071538299.1
• Coordinates: 5472751..5473029 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HBA22_RS25565
• Protein ID: WP_003123432.1
• Coordinates: 5472434..5472739 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HBA22_RS25530 (HBA22_25535)	5467574..5468422	+	849	WP_003123430.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
HBA22_RS25540 (HBA22_25545)	5468589..5469530	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
HBA22_RS25545 (HBA22_25550)	5469647..5470261	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
HBA22_RS25550 (HBA22_25555)	5470303..5470887	+	585	WP_003123431.1	aminoacyl-tRNA hydrolase	-
HBA22_RS25555 (HBA22_25560)	5470928..5472028	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
HBA22_RS25565 (HBA22_25570)	5472434..5472739	-	306	WP_003123432.1	HigA family addiction module antitoxin	Antitoxin
HBA22_RS25570 (HBA22_25575)	5472751..5473029	-	279	WP_071538299.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HBA22_RS25575	5473082..5473210	-	129	Protein_5062	integrase	-
HBA22_RS25580 (HBA22_25580)	5473358..5475586	+	2229	WP_003113525.1	TonB-dependent receptor	-
HBA22_RS25585 (HBA22_25585)	5475656..5476303	-	648	WP_003095021.1	carbonate dehydratase	-
HBA22_RS25590 (HBA22_25590)	5476365..5477603	-	1239	WP_003123433.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10616.17 Da
• Isoelectric Point: 7.8937

>T152310 WP_071538299.1 NZ_CP050149:c5473029-5472751 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHGIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T152310 NZ_CP066108:1685209-1685730 [Enterobacter hormaechei]
GTGGCAAATCTCACGATCGAAATGTTATCAGAGGGAACCGATTACGATTTCGGCAATTTCGATTGTGGTGAACCCTCGCT
CAATGCCTTCCTTACCGATCACCTGGTACGTCAGCACAGCGGACGTATTTTACGCGGCTACCTACTTAAAGAACGCGACC
ACCCACGTATCCTGGGCTATTACACGCTTTCGGGAAGTTGCTTTGAAAAAGCGATGCTTCCTTCAAAGACTCAGCAGCGC
CGCATCCCATACAGCAACGTCCCCAGCGTGACGCTGGGACGTCTGGCTGTCCATAAAGAACTACAGGGAAACGAGTGGGG
AACAACGCTTGTCACACACGCTATGCGCGTTGTTTATCTGGCGTCGCAGGCTGTTGGCGTCCACGGAATTTTTGTGGATG
CGCTTAACGAACGGGCCAAACGATTTTATCTGAAGCTGGGGTTTATCCCGCTGGCAGGTGAAAACAGCAGTTCACTGTTC
TTCCCCACGCAATCTATCGAACGACTGTTTGAGCAGGTGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11204.78 Da
• Isoelectric Point: 4.7769

>AT152310 WP_003123432.1 NZ_CP050149:c5472739-5472434 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAQG

Nucleotide

• Length: 306 bp

>AT152310 NZ_CP066108:1684933-1685202 [Enterobacter hormaechei]
ATGCCAGCACTTAAAAAACAGCGTATCGATCTTCGCTTATCGGACGAAGACAAAACAATGATTGAAGAAGCGGCAGCGAT
GACCAACCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGGGCTGCCGAAGTCATCGAACAGCATCGGCGTC
TCGTATTAAACGAAGCCTCATGGAATGCCGTTATGGATGCCATCGATAATCCGCCAGAGCCAAATGAACGCCTGAAACGC
GCGGCGAAACGTCTTCGAAACATGGAGTGA