Detailed information of TA system TA152304

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5250897..5251492
• Replicon: chromosome
• Accession: NZ_CP050148
• Organism: Pseudomonas aeruginosa strain AA43

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: HBA21_RS24295
• Protein ID: WP_003113526.1
• Coordinates: 5251214..5251492 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HBA21_RS24290
• Protein ID: WP_003099268.1
• Coordinates: 5250897..5251202 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HBA21_RS24255 (HBA21_24305)	5246037..5246885	+	849	WP_003099284.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
HBA21_RS24265 (HBA21_24315)	5247052..5247993	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
HBA21_RS24270 (HBA21_24320)	5248110..5248724	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
HBA21_RS24275 (HBA21_24325)	5248766..5249350	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
HBA21_RS24280 (HBA21_24330)	5249391..5250491	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
HBA21_RS24290 (HBA21_24340)	5250897..5251202	-	306	WP_003099268.1	HigA family addiction module antitoxin	Antitoxin
HBA21_RS24295 (HBA21_24345)	5251214..5251492	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HBA21_RS24300	5251554..5251673	-	120	Protein_4808	integrase	-
HBA21_RS24305 (HBA21_24350)	5251821..5254049	+	2229	WP_021264080.1	TonB-dependent receptor	-
HBA21_RS24310 (HBA21_24355)	5254119..5254766	-	648	WP_003095021.1	carbonate dehydratase	-
HBA21_RS24315 (HBA21_24360)	5254828..5256066	-	1239	WP_019681675.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T152304 WP_003113526.1 NZ_CP050148:c5251492-5251214 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T152304 NZ_CP066104:7386-7802 [Enterobacter hormaechei]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT152304 WP_003099268.1 NZ_CP050148:c5251202-5250897 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT152304 NZ_CP066104:7159-7389 [Enterobacter hormaechei]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: V6ALY3