Detailed information of TA system TA152299

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5786632..5787227
• Replicon: chromosome
• Accession: NZ_CP050147
• Organism: Pseudomonas aeruginosa strain A5803

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HBA20_RS27340
• Protein ID: WP_071535843.1
• Coordinates: 5786949..5787227 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0S3KUN4
• Locus tag: HBA20_RS27335
• Protein ID: WP_003111575.1
• Coordinates: 5786632..5786937 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HBA20_RS27300 (HBA20_27360)	5781772..5782620	+	849	WP_009316211.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
HBA20_RS27310 (HBA20_27370)	5782787..5783728	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
HBA20_RS27315 (HBA20_27375)	5783845..5784459	+	615	WP_003095013.1	50S ribosomal protein L25/general stress protein Ctc	-
HBA20_RS27320 (HBA20_27380)	5784501..5785085	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
HBA20_RS27325 (HBA20_27385)	5785126..5786226	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
HBA20_RS27335 (HBA20_27395)	5786632..5786937	-	306	WP_003111575.1	HigA family addiction module antitoxin	Antitoxin
HBA20_RS27340 (HBA20_27400)	5786949..5787227	-	279	WP_071535843.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HBA20_RS27345	5787280..5787408	-	129	Protein_5418	integrase	-
HBA20_RS27350 (HBA20_27405)	5787556..5789784	+	2229	WP_021264080.1	TonB-dependent receptor	-
HBA20_RS27355 (HBA20_27410)	5789854..5790501	-	648	WP_003095021.1	carbonate dehydratase	-
HBA20_RS27360 (HBA20_27415)	5790563..5791801	-	1239	WP_003111578.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10674.25 Da
• Isoelectric Point: 7.8937

>T152299 WP_071535843.1 NZ_CP050147:c5787227-5786949 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRVGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T152299 NZ_CP066101:2321823-2322314 [Enterobacter hormaechei]
GTGGGACGTGTAACAGCTCCAGAGCCCTTATCCAGTGTTCATCAACTGGCGGAGTTCGTCAGTGGAGAGGCCGTGCTTGA
CGAATGGCTAAAACAAAGAGGCCTGAAAAACCAGGCGCTCGGCGCAGCCCGAACATTTGTGGTTTGTAAAACGGGCACGA
AGCAGGTCGCGGGTTTTTACTCACTGGCTACCGGCAGCGTTAACCATACGGAAGCGACAGGCCATCTTCGCCGTAACATG
CCGGATCCGATTCCCGTGATTGTACTTGCCCGTCTGGCTGTCGATGTCTCATTACACGGAAATGGGTTGGGAGCCGATTT
ACTCCACGATGCGGTTTTACGCTGTTATCGGGTGGCAGAGAATATCGGAGTGAGAGCGATCATGGTTCATGCACTCACTG
AAAACGCCAAAGCTTTCTATCTTCACCACGGTTTTAAAGCATCCCAGACTCAGGAACGAACACTGTTTCTCCGGCTTCCT
CAGGAGAAGTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11205.72 Da
• Isoelectric Point: 4.4475

>AT152299 WP_003111575.1 NZ_CP050147:c5786937-5786632 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAQG

Nucleotide

• Length: 306 bp

>AT152299 NZ_CP066101:2321569-2321835 [Enterobacter hormaechei]
ATGAAATCAGATGTTCAACTCAATATCAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGTACAGACTTTATTCTGGAGATGGCCTGTCAGGCAGCCGAGAATGTGATCCTCGATCGGCGTGTATTCAATT
TTAACGATGAACAGTATGCAGAGTTCATTGACATGCTCGATGCCCCGGTCGCGGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3KUN4