Detailed information of TA system TA152297

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4937132..4937813
• Replicon: chromosome
• Accession: NZ_CP050147
• Organism: Pseudomonas aeruginosa strain A5803

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6AKP0
• Locus tag: HBA20_RS23190
• Protein ID: WP_003111825.1
• Coordinates: 4937448..4937813 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HBA20_RS23185
• Protein ID: WP_003145733.1
• Coordinates: 4937132..4937455 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HBA20_RS23160 (HBA20_23190)	4933045..4933683	+	639	WP_003163192.1	hypothetical protein	-
HBA20_RS23165 (HBA20_23195)	4933926..4934261	+	336	WP_003140884.1	TM2 domain-containing protein	-
HBA20_RS23170 (HBA20_23200)	4934435..4934953	+	519	WP_022579807.1	PAAR domain-containing protein	-
HBA20_RS23175 (HBA20_23205)	4934950..4935651	+	702	WP_033954262.1	VRR-NUC domain-containing protein	-
HBA20_RS23180 (HBA20_23210)	4935668..4936756	+	1089	WP_009315279.1	DUF3396 domain-containing protein	-
HBA20_RS23185 (HBA20_23215)	4937132..4937455	-	324	WP_003145733.1	XRE family transcriptional regulator	Antitoxin
HBA20_RS23190 (HBA20_23220)	4937448..4937813	-	366	WP_003111825.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HBA20_RS23195 (HBA20_23225)	4938077..4938316	-	240	WP_044060930.1	hypothetical protein	-
HBA20_RS23200 (HBA20_23230)	4938523..4938795	+	273	WP_033954259.1	hypothetical protein	-
HBA20_RS23205 (HBA20_23235)	4938826..4939251	-	426	WP_003116492.1	VOC family protein	-
HBA20_RS23210 (HBA20_23240)	4939352..4940236	+	885	WP_033939105.1	LysR substrate-binding domain-containing protein	-
HBA20_RS23215 (HBA20_23245)	4940209..4941162	-	954	WP_003085661.1	LysR substrate-binding domain-containing protein	-
HBA20_RS23220 (HBA20_23250)	4941383..4941817	+	435	WP_003114204.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4923252..4940236	16984

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13880.24 Da
• Isoelectric Point: 4.8219

>T152297 WP_003111825.1 NZ_CP050147:c4937813-4937448 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRHGHMRELRTQHGGRPFRTLYAFDPRRSA
ILLIGGDKTGDDRWYELNVPIADRLYDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T152297 NZ_CP066101:c1225649-1225404 [Enterobacter hormaechei]
CTGGACGATCTTTACCGGGTACTAAAAAAGGAACACTACCCCTCACCAGACGAGATGCGAAAGCGCTTTCCCAGCCTGGA
CCGGATGAAATACCGGGAAAAATGGTGGGTCATTGAAGTGGGTGGCGGGCATCTAAGAGTAATGTTTTTTGCTGATTTTG
AGCGGGGAAAGATCTTCATCAAACACATCAGCACGCACGCAGAGTATGACAAGCTCACGGACTACTACCGGAGAACGAAA
GAATGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11896.73 Da
• Isoelectric Point: 6.4751

>AT152297 WP_003145733.1 NZ_CP050147:c4937455-4937132 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQARGLSQKMLSEVLHVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT152297 NZ_CP066101:c1225407-1224988 [Enterobacter hormaechei]
ATGAATTATGCAGCAGCCATCAAAGCGGCAAACGCTCTGACAAACGAGCTCCCTTTCCTGGGTTCAACCCCGTCGCGGCA
AGACTACGAGGATGCGCTGGCGCTGGTGGAGTATCTTATCGAGCACGACCCGGATAACCCGCTGGTAGATATGCTCACTG
CCAAAATCGATAAGTACGAAAACGAGTCCCCGGAATTTGCAGAATTTAACGCGCGTATTGCCAGTATTCCGTCAGGCGTT
GCGTTACTGCGAACGCTGATGGATCAGTACCAACTCACTCAGAGTGATTTCGAGAACGAGATTGGTAAGAAGTCTCTGGT
GAGTCGGATCCTGAACGGACAGCGCACGTTAACGCTGGATCATATGCGTGCGCTGGCGAAGCGTTTTGGTTTGCCGGTGA
GTGCGTTTGTTGGGAATTAA

Structures

Toxin

• AlphaFold DB: V6AKP0