Detailed information of TA system TA152287

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 3323906..3324447
• Replicon: chromosome
• Accession: NZ_CP050139
• Organism: Komagataeibacter rhaeticus strain ENS 9a1a

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: GWK63_RS15290
• Protein ID: WP_081749560.1
• Coordinates: 3323906..3324175 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A181CES2
• Locus tag: GWK63_RS15295
• Protein ID: WP_061276673.1
• Coordinates: 3324172..3324447 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GWK63_RS15260	3319150..3319704	+	555	WP_007396369.1	hypothetical protein	-
GWK63_RS15265	3319705..3320319	+	615	WP_039998314.1	RES family NAD+ phosphorylase	-
GWK63_RS15270	3320608..3321381	-	774	WP_034931780.1	aromatic-ring-hydroxylating dioxygenase	-
GWK63_RS15275	3321371..3322708	-	1338	WP_172459691.1	transposase	-
GWK63_RS15280	3323068..3323340	-	273	Protein_2999	transposase	-
GWK63_RS17085	3323346..3323893	-	548	Protein_3000	hypothetical protein	-
GWK63_RS15290	3323906..3324175	-	270	WP_081749560.1	Txe/YoeB family addiction module toxin	Toxin
GWK63_RS15295	3324172..3324447	-	276	WP_061276673.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
GWK63_RS15300	3324981..3325339	-	359	Protein_3003	IS110 family transposase	-
GWK63_RS15305	3325352..3326293	-	942	WP_166498167.1	IS21 family transposase	-
GWK63_RS15310	3326420..3326539	+	120	Protein_3005	IS3 family transposase	-
GWK63_RS15315	3326620..3327219	-	600	WP_035366892.1	DUF488 domain-containing protein	-
GWK63_RS15320	3327218..3327433	+	216	WP_061507197.1	DUF2945 domain-containing protein	-
GWK63_RS15330	3327734..3328922	-	1189	Protein_3008	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3183466..3439080	255614

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-89)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-75)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10486.83 Da
• Isoelectric Point: 8.1012

>T152287 WP_081749560.1 NZ_CP050139:c3324175-3323906 [Komagataeibacter rhaeticus]
VKVIFHEDGWEDYLSWFQSDHAVLGKVNALIEDVRRHPFQGIGKPEPLKGQLSGWWSRRITGEHRLVYRVQGRAGEDQRI
EVAQCRFHY

Nucleotide

• Length: 270 bp

>T152287 NZ_CP066094:3080202-3080381 [Enterobacter hormaechei]
ATGGATAGCAGGAATGCAATAGCAATGATAGAAGCCGATGGGTGGTATCTGGTGAGAGTTAAAGGCAGTCATCACCAGTT
CAAACACCCAACGAAAAAGGGGCTGGTAACGGTAAAGCATCCACAGAAAGACATACCGTTACCAACACTGAAAAGCATCA
AAAAACAGGCGGGGCTCTAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10223.41 Da
• Isoelectric Point: 4.8498

>AT152287 WP_061276673.1 NZ_CP050139:c3324447-3324172 [Komagataeibacter rhaeticus]
MTCVSYTDLRQNLAHYLDEAVNSRAPIVVTRKTGKGSVVLMSEEEFSGWQETVHLLSSPRNAERLLRSIRDIEHGAATER
DLLEPQDEPSA

Nucleotide

• Length: 276 bp

>AT152287 NZ_CP066094:3080403-3080807 [Enterobacter hormaechei]
ATGTTGTACCCTGTTGCTATTGATAAAGGCGATTCATCCTTCGGCGTTCGCGTACCTGATATTCCTGGCTGCTTCTCTGG
CGGTGAAGATTATCAGGACGCGATCGAAAGCGTACGTGAAGCGATCGAGGCACACATCGAATTACTGGTTGAAGATGGCG
AGAGCGTTCCCGAAGCAACGACCGTCGAAAACTGGCTATCTGATCCGGAGTACGCCGGTGCGGTATGGGCCCTAGTTGAC
GTGGATATAACACGCCTGATGGGAAAAGCGGAAAAAATCAATGTGACGCTACCCTCATTACTGATCCGACGTATTGATCA
GTTTGTGGCCGCGCATCCTGAATATGGAAGCCGTTCGGGCTTCCTTTCTCGCGTCGCTGCTGACAAGGTTATTAATCAAC
GATAG

Structures

Antitoxin

• AlphaFold DB: A0A181CES2