Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4548635..4549362 Replicon chromosome
Accession NZ_CP050078
Organism Citrobacter koseri strain NJ

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag NCK_RS21490 Protein ID WP_047459826.1
Coordinates 4549054..4549362 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NCK_RS21485 Protein ID WP_199972370.1
Coordinates 4548635..4549054 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NCK_RS21460 (4543831) 4543831..4544760 + 930 WP_219936349.1 1,4-dihydroxy-2-naphthoate polyprenyltransferase -
NCK_RS21465 (4544853) 4544853..4545338 + 486 WP_012133864.1 ribonuclease E activity regulator RraA -
NCK_RS21470 (4545504) 4545504..4545749 - 246 WP_012133867.1 septal ring assembly protein ZapB -
NCK_RS21475 (4546140) 4546140..4546985 + 846 WP_012133869.1 MIP/aquaporin family protein -
NCK_RS21480 (4547009) 4547009..4548517 + 1509 WP_012133870.1 glycerol kinase GlpK -
NCK_RS21485 (4548635) 4548635..4549054 - 420 WP_199972370.1 helix-turn-helix domain-containing protein Antitoxin
NCK_RS21490 (4549054) 4549054..4549362 - 309 WP_047459826.1 type II toxin-antitoxin system HigB family toxin Toxin
NCK_RS21495 (4549503) 4549503..4550513 + 1011 WP_112001490.1 class II fructose-bisphosphatase -
NCK_RS21500 (4550609) 4550609..4551355 + 747 WP_012133874.1 ferredoxin--NADP(+) reductase -
NCK_RS21505 (4551431) 4551431..4551877 - 447 WP_219936351.1 DUF805 domain-containing protein -
NCK_RS21510 (4551978) 4551978..4552574 + 597 WP_047459831.1 YiiQ family protein -
NCK_RS21515 (4552689) 4552689..4553456 + 768 WP_024130662.1 triose-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 12352.15 Da        Isoelectric Point: 10.0486

>T152225 WP_047459826.1 NZ_CP050078:c4549362-4549054 [Citrobacter koseri]
MHVISKEPFEEAAKRYPNDSLAIRALYRLMRETDFSSPTEMRTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHISTHAEYDKLTRYYRENK

Download         Length: 309 bp

>T152225 NZ_CP066058:c2057047-2056742 [Alcaligenes faecalis]
ATGGGAACCATCAAATTACGCAAATGGGATAGTGCCGAATACCTCCAAACAGAGGAAGACATGGTGCTCTACCTGGAAGC
CTGCATGGAAGAAGCGGGCGACGATGCCGCTTTCATTGCAAAAGCATTAGGAAATATTGCTCGCGCTAAAGGCATGAGCC
AACTGGCCAAAGAAACAGGCTTGGGACGTGAAAGCCTGTACAAAGCCCTGTCTGGCGAAGGCAATCCAAGCTTTGGCACC
ATTCTGAAAGTCATGCACGCTTTGGGAATCAAACTGCAACCGCAGCTTGCTCCCCACAACTCCTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15350.35 Da        Isoelectric Point: 4.3485

>AT152225 WP_199972370.1 NZ_CP050078:c4549054-4548635 [Citrobacter koseri]
MNTDTAKAIEATKQLVAAVPFLGGSSSESDYREALSLVDYLIENDDENPLIDFLASKIADYEDNSDRFAEFNKGIAEMPV
GVALLRTLIDQYKLSYSDLKEEIGSKSLVSQILSGQRSLTIAHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT152225 NZ_CP066058:c2057350-2057060 [Alcaligenes faecalis]
ATGAAGACTATCCACACCATCGATACCTTCGATCACTGGTTTGGCGGTCTGCGCGACAAGCAGGCCGCTAGACGGATTCA
GGTTCGTATTGACCGAGCTGAAGATGGCAACTTTGGAGACTGCGAGCCTGTCGGCGAAGGAGTCCCCGAAATGCGTATTC
ACTACGGACCTGGCTACCGTGTGTACTTTGTACAACGTGAACTGGAGATCGTGATCTTATTGGCGGATGGCAATAAATCT
ACCCAGAATAAAGATATTAAAACTGCGCTGGAAATCGCGCGGCAACTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References