Detailed information of TA system TA152222

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2784688..2785422
• Replicon: chromosome
• Accession: NZ_CP050078
• Organism: Citrobacter koseri strain NJ

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A8AI82
• Locus tag: NCK_RS13235
• Protein ID: WP_012132927.1
• Coordinates: 2785117..2785422 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: NCK_RS13230
• Protein ID: WP_198448278.1
• Coordinates: 2784688..2785029 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NCK_RS13205 (2780173)	2780173..2780796	-	624	WP_115609403.1	DsbA family protein	-
NCK_RS13210 (2780793)	2780793..2782679	-	1887	WP_219935641.1	protein-disulfide reductase DsbD family protein	-
NCK_RS13215 (2782728)	2782728..2783093	-	366	WP_047457658.1	hypothetical protein	-
NCK_RS13220 (2783323)	2783323..2784243	+	921	WP_012132923.1	curved DNA-binding protein	-
NCK_RS13225 (2784243)	2784243..2784548	+	306	WP_047457663.1	chaperone modulator CbpM	-
NCK_RS13230 (2784688)	2784688..2785029	-	342	WP_198448278.1	HigA family addiction module antitoxin	Antitoxin
NCK_RS13235 (2785117)	2785117..2785422	-	306	WP_012132927.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
NCK_RS13240 (2785651)	2785651..2786757	+	1107	WP_219935642.1	4Fe-4S binding protein	-
NCK_RS13245 (2786791)	2786791..2787567	-	777	WP_115181740.1	ABC transporter ATP-binding protein	-
NCK_RS13250 (2787567)	2787567..2788337	-	771	WP_047457674.1	ABC transporter permease	-
NCK_RS13255 (2788334)	2788334..2789341	-	1008	WP_049009338.1	ABC transporter substrate-binding protein	-
NCK_RS13260 (2789370)	2789370..2790089	-	720	WP_110494362.1	aspartate/glutamate racemase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (7-101)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11972.70 Da
• Isoelectric Point: 8.3565

>T152222 WP_012132927.1 NZ_CP050078:c2785422-2785117 [Citrobacter koseri]
MPKKLVSIEDFRDQWLDDFFMYSAPHKKIPPDIHSALARKLDIINAAVSYKDLKSPPGNRYEELEGKLQEYSSIRVNKQY
RLIFKWVNGKAQDLYLDPHEY

Nucleotide

• Length: 306 bp

>T152222 NZ_CP066057:c1488785-1488507 [Moraxella nonliquefaciens]
ATGATAAAAAGTTTTAAATGTAAAGATACTAAGACCCTATTTGAAACAGGGGAGAGCCGTGTATTTGGCAACTTTTTATC
GGTCGCAACTCGCAAACTAACCTATCTTGATAATGCTGTAACACTAGATGCCTTACGTTCACCACCAGCCAACCGCTTAG
AGAAACTCGTGGGCGACCGTGAAGGTCAGTACAGCATACGCATTAACGACCAATTTCGCATTTGCTTTGTATGGAGCGAA
GACGGCGTTTATGATGTTGAGATTGTGGATTATCATTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13062.95 Da
• Isoelectric Point: 5.8882

>AT152222 WP_198448278.1 NZ_CP050078:c2785029-2784688 [Citrobacter koseri]
MNQATRKPTTVGDILLYEYLEPLDLKINDLAEMLQVHRNTASALVNNNRKLTTDMAFRLSKVFDTSVEFWMNLQTAVDVW
ETENNARLQVEMSRVITASDFLAERKKQEKKVA

Nucleotide

• Length: 342 bp

>AT152222 NZ_CP066057:c1488497-1488192 [Moraxella nonliquefaciens]
ATGTTTAAAAATGGCATGAGACCTGTACACGCAGGGGAAATTTTGCGTGAAGACTATTTAAAGCCATTAGGCATGTCTGC
CAATGCCCTAGCAAAAGCTCTGGGCGTTACCCCTGCACGCATTAATGACATTGTCCTTGAACGCCGTGGCATTACGGCTG
ATACCGCCTTACGTCTGGCTCGCTATTTTGGGGGTGATGCCCAAACATGGCTAAACTTACAAAGCACTTATGAGTTACGC
CTTGCCGAAAACGAGCTTGGCGACAGCTTGGAGCATATCAGCCCTTATGAAGTTTGTTATACCTAA

Structures

Toxin

• AlphaFold DB: A8AI82