Detailed information of TA system TA152191

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 31716..32446
• Replicon: chromosome
• Accession: NZ_CP050073
• Organism: Enterobacter kobei strain 070

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2W0GI63
• Locus tag: HA514_RS00155
• Protein ID: WP_023331686.1
• Coordinates: 31716..32030 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HA514_RS00160
• Protein ID: WP_023331687.1
• Coordinates: 32027..32446 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HA514_RS00130	27381..28469	+	1089	WP_048997362.1	cellulase family glycosylhydrolase	-
HA514_RS00135	28372..29556	-	1185	WP_047027519.1	multidrug efflux MFS transporter EmrD	-
HA514_RS00140	29732..30565	-	834	WP_121527197.1	DMT family transporter	-
HA514_RS00145	30628..31074	-	447	WP_014881927.1	GNAT family N-acetyltransferase	-
HA514_RS00150	31139..31228	-	90	WP_023331685.1	type I toxin-antitoxin system toxin TisB	-
HA514_RS00155	31716..32030	+	315	WP_023331686.1	type II toxin-antitoxin system HigB family toxin	Toxin
HA514_RS00160	32027..32446	+	420	WP_023331687.1	helix-turn-helix domain-containing protein	Antitoxin
HA514_RS00165	32596..32694	+	99	WP_023331688.1	ilvB operon leader peptide IvbL	-
HA514_RS00170	32801..34489	+	1689	WP_032635253.1	acetolactate synthase large subunit	-
HA514_RS00175	34493..34780	+	288	WP_010426528.1	acetolactate synthase small subunit	-
HA514_RS00180	34863..35456	+	594	WP_014881931.1	transcriptional regulator UhpA	-
HA514_RS00185	35453..36958	+	1506	WP_121527196.1	signal transduction histidine-protein kinase/phosphatase UhpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-136)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12441.59 Da
• Isoelectric Point: 10.2825

>T152191 WP_023331686.1 NZ_CP050073:31716-32030 [Enterobacter kobei]
MHLISMKAILDAVIQFPQHREELLFLGRTIEKSHCTTPVALRKIFPTLDNLKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T152191 NZ_CP066032:2731835-2732233 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15561.18 Da
• Isoelectric Point: 5.6761

>AT152191 WP_023331687.1 NZ_CP050073:32027-32446 [Enterobacter kobei]
MMIVADAMKATHALVAAVPLLGEHPNEKDYQDALELVEYLLMNEPGSPLLDIVCARIRRYEANRPEIVALRQEMESVPVG
IAVLRTLMDQYKLTISDFQDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT152191 NZ_CP066032:2731608-2731835 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Toxin

• AlphaFold DB: A0A2W0GI63