Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 134..673 Replicon plasmid p35_C
Accession NZ_CP050070
Organism Klebsiella aerogenes strain 035

Toxin (Protein)


Gene name yafQ Uniprot ID J2DZM3
Locus tag HA513_RS25935 Protein ID WP_004150484.1
Coordinates 398..673 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID A0A5H6MLU0
Locus tag HA513_RS25930 Protein ID WP_001570852.1
Coordinates 134..394 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HA513_RS25930 134..394 + 261 WP_001570852.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
HA513_RS25935 398..673 + 276 WP_004150484.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
HA513_RS25940 624..830 + 207 WP_162184628.1 hypothetical protein -
HA513_RS25945 842..1498 - 657 WP_166479947.1 hypothetical protein -
HA513_RS25950 1830..2357 + 528 WP_048242344.1 ankyrin repeat domain-containing protein -
HA513_RS25955 2368..2577 + 210 WP_048242343.1 hypothetical protein -
HA513_RS25960 2574..2873 + 300 WP_048242342.1 hypothetical protein -
HA513_RS25965 2870..3163 + 294 WP_048242341.1 hypothetical protein -
HA513_RS25970 3299..3610 + 312 WP_048242340.1 hypothetical protein -
HA513_RS25975 3613..4170 + 558 WP_166479948.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
HA513_RS25980 4186..4725 + 540 WP_045307794.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
HA513_RS25985 4742..4936 + 195 WP_048242338.1 hypothetical protein -
HA513_RS25990 4989..5435 - 447 WP_045351267.1 H-NS histone family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..40745 40745


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-83)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10750.40 Da        Isoelectric Point: 9.6586

>T152190 WP_004150484.1 NZ_CP050070:398-673 [Klebsiella aerogenes]
MTTRTIEYSGQFQKDVKKAQKRHKDMSKLKALMTLLINDALPLPQVYKDHPLQGNYKGYRDAHIEPDWLLIYKITDEVLR
FERTGTHSDLF

Download         Length: 276 bp

>T152190 NZ_CP066032:2352853-2353188 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9239.63 Da        Isoelectric Point: 5.0336

>AT152190 WP_001570852.1 NZ_CP050070:134-394 [Klebsiella aerogenes]
MAANAFVRARIDETLKNEAAVVLAGMGLTVSDLVRITLTKVAKEKALPFDMRVPNELTANTIANSEKGVDVHTAKDADDL
FNQLGI

Download         Length: 261 bp

>AT152190 NZ_CP066032:2352605-2352853 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB J2DZM3

Antitoxin

Source ID Structure
AlphaFold DB A0A5H6MLU0

References