Detailed information of TA system TA152183

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2062805..2063494
• Replicon: chromosome
• Accession: NZ_CP050067
• Organism: Klebsiella aerogenes strain 035

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HA513_RS09995
• Protein ID: WP_042894575.1
• Coordinates: 2063198..2063494 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HA513_RS09990
• Protein ID: WP_032711784.1
• Coordinates: 2062805..2063140 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HA513_RS09970	2058008..2058451	-	444	WP_020078629.1	GNAT family N-acetyltransferase	-
HA513_RS09975	2058632..2059615	+	984	WP_015366313.1	zinc transporter ZntB	-
HA513_RS09980	2060105..2061478	+	1374	WP_042894631.1	ATP-dependent RNA helicase DbpA	-
HA513_RS09985	2061526..2062461	-	936	WP_032711785.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
HA513_RS09990	2062805..2063140	-	336	WP_032711784.1	HigA family addiction module antidote protein	Antitoxin
HA513_RS09995	2063198..2063494	-	297	WP_042894575.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HA513_RS10000	2063731..2064165	-	435	WP_015366316.1	universal stress protein UspF	-
HA513_RS10005	2064310..2065452	-	1143	WP_042894578.1	porin OmpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (7-96)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-84)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11554.16 Da
• Isoelectric Point: 7.4629

>T152183 WP_042894575.1 NZ_CP050067:c2063494-2063198 [Klebsiella aerogenes]
MQNHIGSFRDVWLEEFFLHSAPHKKIPPDIHTTLARKLDIINAAVTYLDLRSPPGNRYEELNGKLEGYSSIRINQQYRLI
FKWVDGKAEDLYLDPHKY

Nucleotide

• Length: 297 bp

>T152183 NZ_CP066032:c574209-573859 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12694.53 Da
• Isoelectric Point: 7.2045

>AT152183 WP_032711784.1 NZ_CP050067:c2063140-2062805 [Klebsiella aerogenes]
MKQATRKPTTVGDILQFEYLEPLDLKINELAEMIHVHRNSVSALVNNNRKLTTDMAFRLSKAFNTTVDFWLNLQAAVDLW
EVENDTRAQEEFSRIVPASKFIASKADKKVA

Nucleotide

• Length: 336 bp

>AT152183 NZ_CP066032:c574454-574203 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA