Detailed information of TA system TA152142

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3660901..3661700
• Replicon: chromosome
• Accession: NZ_CP050047
• Organism: Escherichia coli strain 90-1

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: G9P52_RS17595
• Protein ID: WP_188027863.1
• Coordinates: 3660901..3661365 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: G9P52_RS17600
• Protein ID: WP_001307405.1
• Coordinates: 3661365..3661700 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G9P52_RS17565	3655902..3656336	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
G9P52_RS17570	3656354..3657232	-	879	WP_001298758.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
G9P52_RS17575	3657222..3658001	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
G9P52_RS17580	3658012..3658485	-	474	WP_188027862.1	PTS N-acetylgalactosamine transporter subunit IIB	-
G9P52_RS17585	3658508..3659788	-	1281	WP_000681947.1	tagatose-bisphosphate aldolase subunit KbaZ	-
G9P52_RS17590	3660037..3660846	+	810	WP_000072180.1	aga operon transcriptional regulator AgaR	-
G9P52_RS17595	3660901..3661365	-	465	WP_188027863.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
G9P52_RS17600	3661365..3661700	-	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
G9P52_RS17605	3661849..3663420	-	1572	WP_001273741.1	galactarate dehydratase	-
G9P52_RS17610	3663795..3665129	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
G9P52_RS17615	3665145..3665915	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17837.19 Da
• Isoelectric Point: 9.4947

>T152142 WP_188027863.1 NZ_CP050047:c3661365-3660901 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKATTKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T152142 NZ_CP065997:6020336-6020647 [Achromobacter deleyi]
ATGAGCAAGAAGACGATCAAGGTTTCCGAACTGCCCACCTTCGACCCTGCGCGTTACCTGCGCGACGACAAGGACATCGC
CGCCTACTTGACGCAGGTGATCGAGGACGGAGACTTCGGTGAGCTTGCCCAGGCGCTGGGAGTTGCCGCGCGCGCTCGCG
GCATGACGCAAATTGCCGAGACTGCCGGTATTGGCCGGGAATCCCTGTATAAGGCCCTGCGGGCTGGCGCCAGCCCGCGC
TTTGACACCATCGCCAAGGTATGCAAGGCACTGGGCGTACGCCTTGTCGTCCAGGCCGAACATCCGGCCTAG

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT152142 WP_001307405.1 NZ_CP050047:c3661700-3661365 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT152142 NZ_CP065997:6020025-6020339 [Achromobacter deleyi]
ATGTACCACATAAAGCCGCTGCCAGAGTTCGACGCTTGGTTCGACACCCTGGCAGACCCGGCTACGCGAGCCCGCCTGCT
CCGCAGGCTTGAAAAGGCCGCGCGGGGTTTGCTGGGCGATGTGAAACCGGTCGGAGAGGGCGTGAGCGAGATGCGCGAGT
TCTTTGGCCCTGGCTGGCGCACGTATTACACCGAGCAGGGCGACGTGCTGATCGTCATGCTGGGCGGCGGCACACAGGCC
ACACAAAGCCGTGACGTCAAGCACGCCAAGGAATTGGCGCGGGCTCTGAGAAACGACGAGGAAGACCAATCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0XVC7