Detailed information of TA system TA152138

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2828777..2829379
• Replicon: chromosome
• Accession: NZ_CP050047
• Organism: Escherichia coli strain 90-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: G9P52_RS13590
• Protein ID: WP_000897305.1
• Coordinates: 2829068..2829379 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G9P52_RS13585
• Protein ID: WP_000356397.1
• Coordinates: 2828777..2829067 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G9P52_RS13560	2824324..2824959	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
G9P52_RS13565	2824956..2825885	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
G9P52_RS13570	2826284..2827264	+	981	WP_000399648.1	IS110-like element IS621 family transposase	-
G9P52_RS13575	2827493..2827735	-	243	WP_001086388.1	hypothetical protein	-
G9P52_RS13580	2827954..2828172	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
G9P52_RS13585	2828777..2829067	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
G9P52_RS13590	2829068..2829379	-	312	WP_000897305.1	hypothetical protein	Toxin
G9P52_RS13595	2829608..2830516	+	909	WP_001162704.1	alpha/beta hydrolase	-
G9P52_RS13600	2830580..2831521	-	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
G9P52_RS13605	2831566..2832003	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
G9P52_RS13610	2832000..2832872	-	873	WP_000920762.1	virulence factor BrkB family protein	-
G9P52_RS13615	2832866..2833465	-	600	WP_001295269.1	glucose-1-phosphatase	-
G9P52_RS13620	2833564..2834349	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2826284..2827264	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T152138 WP_000897305.1 NZ_CP050047:c2829379-2829068 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T152138 NZ_CP065990:59363-59548 [Veillonella parvula]
TTGACTACACCTAAAGAATTGATGAAGGATTTAAAAAAAGATGGTTGGTATATTGATAGAATTAAAGGCTCGCATCATAT
ACTAAAGCATCCAATAAAAGCTGGTCGTGTAACGATTCCGCTACATAAGGAAGATTTAAAGCCTAAAACATTACAAACAA
TCTTGAAACAAGCGGGGCTTAAATAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT152138 WP_000356397.1 NZ_CP050047:c2829067-2828777 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT152138 NZ_CP065990:59617-60015 [Veillonella parvula]
ATGAGAAAGGAAATGTACCCAGCTATCTTTAGTACAGATGGTAAAAATGGATATACGGTAAGTTTTCCAGATTTATTAGG
CTGTGTAACTGAAGGCGATACTTTATGTGAAGCCGTAGAGATGGCTGAAGATGCACTAGGTATTTACTTGTATTCATTGA
GTGAAGATGGCGAGGAATTTCCAGAAGCAAGTAATCCAATCGATATTAAATGTAATAAAGGTGAATTCATAGATTTAGTA
AAATGGGATGAAGAGGAATATTTAAAAAGAACCGATAATAAAGCTGTAAAAAAGACCCTTACAATACCAAGCTGGTTAAA
TCATAAAGCTGAAGAGAAGCAACTTAATTTTTCACAAATTTTACAGAGAGCATTAAAGCAAGAGTTAGAATTAGTATAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7