Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 100952..101454 Replicon chromosome
Accession NZ_CP050047
Organism Escherichia coli strain 90-1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag G9P52_RS00480 Protein ID WP_000767829.1
Coordinates 101200..101454 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag G9P52_RS00475 Protein ID WP_001259255.1
Coordinates 100952..101203 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9P52_RS00450 96130..97197 - 1068 WP_000080117.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
G9P52_RS00455 97197..98267 - 1071 WP_001099214.1 histidinol-phosphate transaminase -
G9P52_RS00460 98264..99568 - 1305 WP_001358504.1 histidinol dehydrogenase -
G9P52_RS00465 99574..100473 - 900 WP_000131792.1 ATP phosphoribosyltransferase -
G9P52_RS00470 100619..100669 - 51 WP_001364200.1 his operon leader peptide -
G9P52_RS00475 100952..101203 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
G9P52_RS00480 101200..101454 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
G9P52_RS00485 101537..102361 + 825 WP_000754758.1 SDR family oxidoreductase -
G9P52_RS00490 102407..103336 + 930 WP_000803351.1 LysR family transcriptional regulator -
G9P52_RS00495 103551..103613 + 63 WP_010723108.1 membrane protein YoeI -
G9P52_RS00500 103603..104961 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
G9P52_RS00505 105140..106198 + 1059 WP_000492340.1 transport protein YeeE -
G9P52_RS00510 106212..106439 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T152121 WP_000767829.1 NZ_CP050047:101200-101454 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T152121 NZ_CP065970:3080354-3080457 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT152121 WP_001259255.1 NZ_CP050047:100952-101203 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT152121 NZ_CP065970:c3080495-3080224 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References