Detailed information of TA system TA152116

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3705161..3705855
• Replicon: chromosome
• Accession: NZ_CP050044
• Organism: Escherichia coli strain 74-2

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: G9P51_RS17770
• Protein ID: WP_001263493.1
• Coordinates: 3705161..3705559 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: G9P51_RS17775
• Protein ID: WP_000554757.1
• Coordinates: 3705562..3705855 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G9P51_RS17740	3700161..3701405	-	1245	WP_000189541.1	esterase FrsA	-
-	3700821..3700901	-	81	NuclAT_11	-	-
-	3700821..3700901	-	81	NuclAT_11	-	-
-	3700821..3700901	-	81	NuclAT_11	-	-
-	3700821..3700901	-	81	NuclAT_11	-	-
G9P51_RS17745	3701497..3701955	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
G9P51_RS17750	3702216..3703673	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
G9P51_RS17755	3703730..3704251	-	522	Protein_3470	peptide chain release factor H	-
G9P51_RS17760	3704250..3704453	-	204	Protein_3471	RNA ligase RtcB family protein	-
G9P51_RS17765	3704699..3705151	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
G9P51_RS17770	3705161..3705559	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
G9P51_RS17775	3705562..3705855	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
G9P51_RS17780	3705907..3706962	-	1056	WP_001226164.1	DNA polymerase IV	-
G9P51_RS17785	3707033..3707956	-	924	WP_001468020.1	putative lateral flagellar export/assembly protein LafU	-
G9P51_RS17790	3707959..3708822	-	864	WP_182123271.1	flagellar motor stator protein MotA	-
G9P51_RS17795	3708835..3709554	-	720	WP_000938740.1	FliA/WhiG family RNA polymerase sigma factor	-
G9P51_RS17800	3709574..3710041	-	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T152116 WP_001263493.1 NZ_CP050044:c3705559-3705161 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T152116 NZ_CP065970:2137392-2137703 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT152116 WP_000554757.1 NZ_CP050044:c3705855-3705562 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT152116 NZ_CP065970:2137700-2138119 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6