Detailed information of TA system TA152096

Bioinformatically predicted

Overview

TA module

• Type: V
• Classification (family/domain): ghoTS/ghoT-GhoS
• Location: 4046707..4047204
• Replicon: chromosome
• Accession: NZ_CP050040
• Organism: Escherichia coli strain 63-2

Toxin (Protein)

• Gene name: ghoT
• Uniprot ID: L4JJG6
• Locus tag: G9P50_RS19350
• Protein ID: WP_001173343.1
• Coordinates: 4046707..4046880 (-)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: ghoS
• Uniprot ID: L4JK92
• Locus tag: G9P50_RS19355
• Protein ID: WP_000398619.1
• Coordinates: 4046908..4047204 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G9P50_RS19340	4043372..4044829	+	1458	WP_000856829.1	dipeptide/tripeptide permease DtpC	-
G9P50_RS19345	4045066..4046583	+	1518	WP_001295074.1	lysine--tRNA ligase	-
G9P50_RS19350	4046707..4046880	-	174	WP_001173343.1	type V toxin-antitoxin system toxin GhoT	Toxin
G9P50_RS19355	4046908..4047204	-	297	WP_000398619.1	type V toxin-antitoxin system endoribonuclease antitoxin GhoS	Antitoxin
G9P50_RS19360	4047431..4047703	-	273	WP_000405647.1	N-acetyltransferase	-
G9P50_RS19365	4047715..4047945	-	231	WP_000371704.1	(4Fe-4S)-binding protein	-
G9P50_RS19370	4048126..4049757	+	1632	WP_001216477.1	sensor histidine kinase	-
G9P50_RS19375	4049754..4050473	+	720	WP_000611288.1	two-component system response regulator DcuR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View HG738867 Escherichia coli str. K-12 substr. MC4100 Genomic island TA020087 4238430..4247780 0.002 View NC_007779 Escherichia coli str. K-12 substr. W3110 Genomic island TA021458 4357262..4366612 0.002 View NC_012759 Escherichia coli BW2952 Genomic island TA023874 4289342..4298692 0.002 View NZ_HG738867 Escherichia coli str. K-12 substr. MC4100 strain K-12 Genomic island TA285032 4238430..4247780 0.002	inside	Genomic island	-	-	4037849..4047204	9355

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-57)

Antitoxin

• Endoribonuclease antitoxin GhoS (5-94)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6555.06 Da
• Isoelectric Point: 10.2288

>T152096 WP_001173343.1 NZ_CP050040:c4046880-4046707 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Nucleotide

• Length: 174 bp

>T152096 NZ_CP065965:c4824455-4823133 [Stenotrophomonas maltophilia]
ATGGGTACGCTGCAGGTCTGGATGAATGGTGTGGAAGTCGCACTTTGGGATGATGTCCGGAACGGGTCATCCCGGTTGTC
GTATGTCAGCCAGTGGATCAGCTCGCCCCAGTCCCGACCGTTGTCGCTCTCTCTACCCCTGCTCCCAGATGGCGAGAGCC
ATCGGGGCAACGTCGTCAACGACTACTTCGATAACCTTCTTCCCGATAACGTCGCCATACGCAACCGGATCCGCGACCGG
TTCGCCACGCGCGGCAGCGATACGTTCGATCTTCTTGAAGCCATTGGACGGGATTGTGTCGGCGCAGTGCAGCTGTTGCC
GCCAGCGCATCCACCCGAAGACATCCAGCAGATCACGTGCGAGGTGTTGGACGAAGCAGGTGTCGCTACGATCCTGCGTC
AGGTGACGACAGGGAACCTGCCTGGCGCACCCAGCCGTGATGATGCATTCCGCATTTCCATCGCCGGTGCGCAGGAAAAG
ACCGGTCTTCTGCTGCATGCTGGCAAATGGTGCATTCCTACCGGCAACACACCATCCACTCACATCTTCAAGCTGCCGCT
TGGCATTGTTGGCAACATGCGGGCAGACATGCATCAGTCCGTGGAGAATGAATGGCTGTGCATGCATCTGCTTTCCGCCT
TTGATATCCCCGTGGCCGAGACGCAGATGGGCCGCTTCAACGACCAACGTGCTCTCATTGTCGAGCGGTTTGACCGTCGC
CTGTCTGCCGACCGCCGGTGGTGGCTGCGACTGCCACAGGAAGACATGTGTCAGGTCTTTGGTCTGCCGGCTGCCAGAAA
GTATGAATCCGATGGCGGCCCCGGTATCGTTCCGATCATGGATCTGCTTCGACAGTCCGAACACCCCGAGGATCGCGCGA
TCTTCTTCAAGACGCAGATTCTTTTCTGGATGCTGGCCGCAACCGACGGCCATGCCAAGAATTTCAGTGTTCACTTGGAA
GCCGGTGGACGCTTCCGCCTGACGCCCGTCTACGACGTGCTGTCGGTATATCCCATCCTCGGCAGGGGACCCAATCAACT
CGATCCACGAAACGCACAGCTCGCCATGGCCGTACATGGCAAGAACCGCCACCGGAAACTGCACCTTGTGCGACGGCGGC
ACTGGAATGAAACCGCGCGCGCCTGCGGCGTTGCCGATGGTGCAGAACCATGGATCAAGACATTGATCGCGGCCGTCGAG
CCCGCCATCCAGCAGGTCACCCGTCAGCTCCCGGATGACTTCCCCTCAAACGTGTCCGAACCGATTTTCAACGGACTTCG
TGCTGCGGCAGCACGCCTTTCCAGCATGGCCCCCGAGGCTTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11467.97 Da
• Isoelectric Point: 4.1705

>AT152096 WP_000398619.1 NZ_CP050040:c4047204-4046908 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Nucleotide

• Length: 297 bp

>AT152096 NZ_CP065965:c4824716-4824459 [Stenotrophomonas maltophilia]
ATGGACAGGCCGCTGCACACAGTGAGGACAGCCCAACAACTTGCGCCCCTGATGCGTGCTTTCCGGCGCCAGGCGGGCCT
GAGCCAGGCTCAGCTGGCGGAGCGACTCGGGATCAGCCGCCAGGCAGTGGGCGCGCTGGAACGCGACCCTGCATCGGCAA
GTTTTGAACGCCTGATGCGGATCTGGGCGGTGCTGGGTCTGGAGGTCAGCCTCCAGCAACGAAGTCCCCAGGAGAACACG
TCGTCATTGGAGTGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QI41

Antitoxin

• AlphaFold DB: A0A7U9QBH6