Detailed information of TA system TA152092

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3538638..3539332
• Replicon: chromosome
• Accession: NZ_CP050040
• Organism: Escherichia coli strain 63-2

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: G9P50_RS16890
• Protein ID: WP_001263493.1
• Coordinates: 3538638..3539036 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: -
• Locus tag: G9P50_RS16895
• Protein ID: WP_152927302.1
• Coordinates: 3539039..3539332 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	3533696..3533776	-	81	NuclAT_8	-	-
-	3533696..3533776	-	81	NuclAT_8	-	-
-	3533696..3533776	-	81	NuclAT_8	-	-
-	3533696..3533776	-	81	NuclAT_8	-	-
G9P50_RS16860	3534372..3534830	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
G9P50_RS16865	3535091..3536548	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
G9P50_RS16870	3536605..3537126	-	522	Protein_3296	peptide chain release factor H	-
G9P50_RS16875	3537125..3537328	-	204	Protein_3297	RNA ligase RtcB family protein	-
G9P50_RS16880	3537586..3538283	-	698	Protein_3298	IS1 family transposase	-
G9P50_RS16885	3538338..3538628	-	291	Protein_3299	GNAT family N-acetyltransferase	-
G9P50_RS16890	3538638..3539036	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
G9P50_RS16895	3539039..3539332	-	294	WP_152927302.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
G9P50_RS16900	3539384..3540439	-	1056	WP_001226164.1	DNA polymerase IV	-
G9P50_RS16905	3540510..3541295	-	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
G9P50_RS16910	3541240..3542979	+	1740	WP_152927301.1	flagellar biosynthesis protein FlhA	-
G9P50_RS16915	3543203..3543700	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	gmhA/lpcA	3519067..3560463	41396
	-	flank	IS/Tn	-	-	3537586..3538089	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T152092 WP_001263493.1 NZ_CP050040:c3539036-3538638 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T152092 NZ_CP065960:349420-349527 [Mammaliicoccus sciuri]
ATGGTTCAAGACCTAGAAGATGTTAAAGAACTAGGTAAAGAAATGGAACAAATATCAGAACAAAATGATGAAGCTTTTGA
AGAAAAGAAATCTGAACAAGAATCTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11180.76 Da
• Isoelectric Point: 5.2168

>AT152092 WP_152927302.1 NZ_CP050040:c3539332-3539039 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKACFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT152092 NZ_CP065960:348777-349376 [Mammaliicoccus sciuri]
ATGGCTTTAAACTTTAAAGTATTTGAAACAAAAGATATTGCTGCACGATATGCTGCAGATGTAGTTCGCAAACAATTTAA
TTCAGACAGCACTTCAGTTGTCGGCGTACATTTAGAAGCAGAGCAAACATCATTTTTCGATGAATTATTAGATGATGTTA
AGAAAAATCCTGTGAACTTCAGTCAAATTCATATTTTAGACTTTGATGGTAAAGAAGATTATTACACAGAATTAGGTGTT
CCTGCTAAACAAGTATTAAAAACTGATGTCGGTAGTGATGTTGAAGCATTTATTAAAGAAAAAGTGAATACTGATAAAAA
GAAAAACAAATTGATGTTAGATGTTGTCACTTTAACTGAAGATAGCAGAACTGGATTAGACTATGACCAAGCAATTCATC
CAGCAAGAGAAGTTATCGTACTTGTCACTGGTAAAGAAAAAGCTAAAGCAGTAAGTCAGTTATATCAAGAACCTGCAAAT
GGTTCTGTTGAATCCGCTAAATTAAAACAACATAGAATGGTAACAGTTGTATTAGATAATGAAGCGGCACAAGGGTTACC
TGAAGACGTTAGATCTTACTTCTCAATTCAATTTTCATAA

Structures

Toxin

• AlphaFold DB: S1EXB8