Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1753964..1754466 Replicon chromosome
Accession NZ_CP050040
Organism Escherichia coli strain 63-2

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag G9P50_RS08235 Protein ID WP_000767829.1
Coordinates 1754212..1754466 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag G9P50_RS08230 Protein ID WP_001259255.1
Coordinates 1753964..1754215 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9P50_RS08205 1749142..1750209 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
G9P50_RS08210 1750209..1751279 - 1071 WP_001468894.1 histidinol-phosphate transaminase -
G9P50_RS08215 1751276..1752580 - 1305 WP_032214175.1 histidinol dehydrogenase -
G9P50_RS08220 1752586..1753485 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
G9P50_RS08225 1753631..1753681 - 51 WP_001364200.1 his operon leader peptide -
G9P50_RS08230 1753964..1754215 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
G9P50_RS08235 1754212..1754466 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
G9P50_RS08240 1754549..1755373 + 825 WP_000754749.1 SDR family oxidoreductase -
G9P50_RS08245 1755419..1756348 + 930 WP_000803351.1 LysR family transcriptional regulator -
G9P50_RS08250 1756563..1756625 + 63 WP_010723108.1 membrane protein YoeI -
G9P50_RS08255 1756615..1757973 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
G9P50_RS08260 1758152..1759210 + 1059 WP_053276361.1 transport protein YeeE -
G9P50_RS08265 1759224..1759451 + 228 WP_000985280.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T152079 WP_000767829.1 NZ_CP050040:1754212-1754466 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T152079 NZ_CP065949:2052201-2052303 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT152079 WP_001259255.1 NZ_CP050040:1753964-1754215 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT152079 NZ_CP065949:c2052310-2052165 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References