Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1825864..1826366 Replicon chromosome
Accession NZ_CP050036
Organism Escherichia coli strain 58-3

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag G9P49_RS08685 Protein ID WP_000767829.1
Coordinates 1826112..1826366 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag G9P49_RS08680 Protein ID WP_001259255.1
Coordinates 1825864..1826115 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9P49_RS08655 1821042..1822109 - 1068 WP_000080085.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
G9P49_RS08660 1822109..1823179 - 1071 WP_000108980.1 histidinol-phosphate transaminase -
G9P49_RS08665 1823176..1824480 - 1305 WP_001298841.1 histidinol dehydrogenase -
G9P49_RS08670 1824486..1825385 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
G9P49_RS08675 1825531..1825581 - 51 WP_001368802.1 his operon leader peptide -
G9P49_RS08680 1825864..1826115 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
G9P49_RS08685 1826112..1826366 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
G9P49_RS08690 1826449..1827273 + 825 WP_000754737.1 SDR family oxidoreductase -
G9P49_RS08695 1827319..1828248 + 930 WP_000803351.1 LysR family transcriptional regulator -
G9P49_RS08700 1828463..1828525 + 63 WP_010723108.1 membrane protein YoeI -
G9P49_RS08705 1828515..1829873 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
G9P49_RS08710 1830052..1831110 + 1059 WP_000492315.1 transport protein YeeE -
G9P49_RS08715 1831124..1831351 + 228 WP_000985280.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T152056 WP_000767829.1 NZ_CP050036:1826112-1826366 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T152056 NZ_CP065946:c1250678-1250373 [Pectobacterium parmentieri]
ATGCAATTCATTGTTTATGAATACAAACGATCCAGTCACTACAAAATATTTGTCGATGTGCAGAGTGATATTGTCGACAC
ACCCAAGCGGCGCATGGTCATTCCGCTTATCGAATCACATCACCTGTCTGAGAAAGTGAATAAAACATTGTTCCCTCAGA
TCCGCATCGACGGCGAAGATTATCGGCTGATGACCACTGAGCTATCGAGCGTTCCCGTTAAGGTGATTGGTGGAGAAATC
GCGGCTCTTGGTGACTACGCTGATGAGATTAAGGATGCTATTAATCTTATGTTTTGGGGGATTTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT152056 WP_001259255.1 NZ_CP050036:1825864-1826115 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT152056 NZ_CP065946:c1250899-1250681 [Pectobacterium parmentieri]
ATGAAACACCGCGTCAGCGTTACCGTGGACAAAGACAGTTATCAGGTTCTGAGTGCTGCCGGAGTCAATATTTCCGGGCT
GGTGAACGATGCCATTGGCAAAGAAGCCCGCCGTATCAAGGCTGAAGAATGGAAGAAGGAAAACCGCGAAGGGATGGAAG
AAGTCGCCCGATTTATCGCACAGCATGGCTCCTTTGCAGATGAAAACAGGAATTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References