Detailed information of TA system TA152053

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 706674..707367
• Replicon: chromosome
• Accession: NZ_CP050036
• Organism: Escherichia coli strain 58-3

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: G9P49_RS03430
• Protein ID: WP_000415584.1
• Coordinates: 706674..706970 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: G9P49_RS03435
• Protein ID: WP_000650107.1
• Coordinates: 706972..707367 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G9P49_RS03395	701762..702076	-	315	WP_000958598.1	antibiotic biosynthesis monooxygenase	-
G9P49_RS03400	702107..702688	-	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
G9P49_RS03405	703007..703339	+	333	WP_000917684.1	DUF2645 family protein	-
G9P49_RS03410	703385..704734	-	1350	WP_000673402.1	two-component system sensor histidine kinase QseC	-
G9P49_RS03415	704731..705390	-	660	WP_001221493.1	two-component system response regulator QseB	-
G9P49_RS03420	705542..705934	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
G9P49_RS03425	705987..706469	+	483	WP_000183505.1	transcriptional regulator	-
G9P49_RS03430	706674..706970	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
G9P49_RS03435	706972..707367	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
G9P49_RS03440	707500..709107	+	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
G9P49_RS03445	709245..711503	+	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T152053 WP_000415584.1 NZ_CP050036:706674-706970 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T152053 NZ_CP065940:c229135-228848 [Enterobacter hormaechei]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT152053 WP_000650107.1 NZ_CP050036:706972-707367 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT152053 NZ_CP065940:c229374-229135 [Enterobacter hormaechei]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65