Detailed information of TA system TA152026

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4542945..4543675
• Replicon: chromosome
• Accession: NZ_CP050013
• Organism: Serratia marcescens strain BP2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8B4GR63
• Locus tag: HAP49_RS21605
• Protein ID: WP_004937033.1
• Coordinates: 4542945..4543256 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HAP49_RS21610
• Protein ID: WP_033632013.1
• Coordinates: 4543253..4543675 (+)
• Length: 141 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HAP49_RS21575	4538272..4538997	+	726	WP_004937040.1	LPS export ABC transporter ATP-binding protein	-
HAP49_RS21580	4539050..4540483	+	1434	WP_004937039.1	RNA polymerase factor sigma-54	-
HAP49_RS21585	4540507..4540794	+	288	WP_004937038.1	ribosome hibernation promoting factor	-
HAP49_RS21590	4540950..4541411	+	462	WP_016930212.1	PTS IIA-like nitrogen regulatory protein PtsN	-
HAP49_RS21595	4541539..4542393	+	855	WP_015379177.1	RNase adapter RapZ	-
HAP49_RS21600	4542390..4542662	+	273	WP_004937034.1	PTS phosphocarrier protein NPr	-
HAP49_RS21605	4542945..4543256	+	312	WP_004937033.1	type II toxin-antitoxin system HigB family toxin	Toxin
HAP49_RS21610	4543253..4543675	+	423	WP_033632013.1	helix-turn-helix domain-containing protein	Antitoxin
HAP49_RS21615	4543679..4545595	-	1917	WP_015379179.1	BglG family transcription antiterminator	-
HAP49_RS21620	4545647..4546861	-	1215	WP_166445425.1	lactonase family protein	-
HAP49_RS21625	4546949..4547689	-	741	WP_033632010.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (81-135)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12289.14 Da
• Isoelectric Point: 10.0957

>T152026 WP_004937033.1 NZ_CP050013:4542945-4543256 [Serratia marcescens]
MHIISREPFNNATKQYPNEATALEAIYKALRRGEFRTPDELKALFPSLDRMKYKEKWWVIDVGGNHLRILFFASFETQKV
FIKHIVSHAEYDKLMQHYRRSSS

Nucleotide

• Length: 312 bp

>T152026 NZ_CP065925:757810-758163 [Staphylococcus pseudintermedius]
ATGAAGCGCGGAGACGTTTATTTAGCAGATTTATCACCAGTACAGGGCTCTGAACAGGGGGGAGTAAGGCCCGTTGTCAT
TATTCAAAATGACACTGGTAATAAGTATAGTCCCACTGTGATTGTAGCCGCAATTACTGGTCGGATTAATAAGGCAAAGA
TACCAACGCATGTCGAAATCGAAAAAAATAAATATAAGCTCGATAAAGATTCTGTTATTTTACTAGAACAAATTCGAACA
GTCGATAAGAAACGATTAAAAGAAAAGTTAACGTTTTTGTCTGAGAAGAAGATGAAAGAAGTTAACAATGCGATTGGTAT
TAGTTTAGGACTGCACATGAATCATCATAAATAG

Antitoxin

Protein

• Length: 141 a.a.
• Molecular weight: 15833.20 Da
• Isoelectric Point: 5.2297

>AT152026 WP_033632013.1 NZ_CP050013:4543253-4543675 [Serratia marcescens]
MITDAIKAADALLRAVPLLQGSRAQQDYREALELVEYLLENDERHPLIDMLAKKIAEYEDNSAEFTAFNQRITQLPQGVA
ALRVLMEQHQLSQSDFEHEIGKKSLVNQILNGKRSLTIPHIMALAKRFNLPPALFLPEAD

Nucleotide

• Length: 423 bp

>AT152026 NZ_CP065925:757639-757809 [Staphylococcus pseudintermedius]
ATGTCAGCATTCAATCAAACAAGATTTAAAAATTTAGAACAGTCGTTAAAAGAAGGTTATGTACAGATGGCAGATTTAAA
TCTCTCCCTAGCCACTGAGGCGTATCCAGTTGAATGTGAAGCTTGCGATTGTAATGAATCACACTTGACAACTGATTACA
AGGAAGACTAA

Structures

Toxin

• AlphaFold DB: A0A8B4GR63