Detailed information of TA system TA152003

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4691443..4692259
• Replicon: chromosome
• Accession: NZ_CP050009
• Organism: Citrobacter sp. Y3

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: HAP28_RS22755
• Protein ID: WP_109739561.1
• Coordinates: 4691443..4691919 (-)
• Length: 159 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: A0A3Q8DNJ3
• Locus tag: HAP28_RS22760
• Protein ID: WP_042999108.1
• Coordinates: 4691924..4692259 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HAP28_RS22735	4686548..4687291	-	744	WP_044264050.1	molecular chaperone	-
HAP28_RS22740	4687334..4689808	-	2475	WP_044257386.1	fimbria/pilus outer membrane usher protein	-
HAP28_RS22745	4689872..4690321	-	450	WP_086513030.1	type 1 fimbrial protein	-
HAP28_RS22750	4690489..4691019	-	531	WP_042999110.1	type 1 fimbrial protein	-
HAP28_RS22755	4691443..4691919	-	477	WP_109739561.1	type II toxin-antitoxin system YhaV family toxin	Toxin
HAP28_RS22760	4691924..4692259	-	336	WP_042999108.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
HAP28_RS22765	4692474..4693010	-	537	WP_042999107.1	ferredoxin-type protein NapF	-
HAP28_RS22770	4693007..4693603	-	597	WP_166444275.1	molecular chaperone	-
HAP28_RS22775	4693648..4694421	-	774	WP_044327723.1	dimethyl sulfoxide reductase anchor subunit	-
HAP28_RS22780	4694414..4695040	-	627	WP_109739562.1	dimethylsulfoxide reductase subunit B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (15-110)

Sequences

Toxin

Protein

• Length: 159 a.a.
• Molecular weight: 18170.78 Da
• Isoelectric Point: 8.2616

>T152003 WP_109739561.1 NZ_CP050009:c4691919-4691443 [Citrobacter sp. Y3]
MDFPTWLNGWTIYAHPCFIDQYNALVARVEDLKHRFPDPIVFQKKKETMVLAHLLKSIANITHEPRASVYRPGDSIGKAY
TDWSRAKFGGGRYRLFFRYSLESKIIVIAWVNDEGSLRTCGSKTDAYKIFGKMLDEGNPPDDWLSLLQACQNDGKEHL

Nucleotide

• Length: 477 bp

>T152003 NZ_CP065905:5993-6268 [Paracoccus marcusii]
GTGCCTGAACTCGAATGGAAGGCCGCGGCGGTCGCGGATCTGATGGCGATTGTCGATTACATTTCGGACGACAACCCAAA
CGCCGCTCTTGCCTTGATGGAGGGCATTCAAAGCAAGGTGGCACAGCTTCCGGCGCATCCGAAACGCTGCCGCCCCGGCA
GGGTGGAGGGCACGCGGGAGCTGGTGGTCCGACCCAACTACATCGTCGTCTATGCCGAAACTGCAGCGGCGGTCACGGTG
CTGCGCGTGCTTCACGCCGCGCAGATGTGGCCCTAG

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12505.83 Da
• Isoelectric Point: 4.1876

>AT152003 WP_042999108.1 NZ_CP050009:c4692259-4691924 [Citrobacter sp. Y3]
MFAYVRSEDIAIEADSKLTERHQTTIPASIRDALHLRSGDRIHYTLLSTGEVLLSKYSEATEDGVMRSFLDFLSQDVANN
PQTLKKLDLSRGYELVDGIDVNLDDEISDED

Nucleotide

• Length: 336 bp

>AT152003 NZ_CP065905:5707-6000 [Paracoccus marcusii]
ATGCCTGCCCAAACTTCGATGCTCCATGTTCGCGTTGATGACCAGCTCAAGGCACAAGCAACCGCCGCCCTGTCTGGCGT
TGGTCTGACGCTCTCGGATGCTGTACGCATCCTGCTGACCCGTGTGGCTGCCGAAGGTGGCTTGCCGGCTGGTCTGACAG
TGGACCCCGCTGCTTACGACACTTGGTTTCGCACTAAGGTTCAGGAGGCTCTGGCCGATCCCCGCCCGGCTACCCCGCAC
GCTCAGGTCATGCAGGAAGCCCGCGCCCTGATCGACGGAAAGCGGCGTGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A3Q8DNJ3