Detailed information of TA system TA151990

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2540860..2541422
• Replicon: chromosome
• Accession: NZ_CP050009
• Organism: Citrobacter sp. Y3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8B3ZQU4
• Locus tag: HAP28_RS12025
• Protein ID: WP_044267611.1
• Coordinates: 2540860..2541138 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HAP28_RS12030
• Protein ID: WP_043000693.1
• Coordinates: 2541138..2541422 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HAP28_RS12005	2535985..2536416	-	432	WP_043000697.1	peroxiredoxin OsmC	-
HAP28_RS12010	2536644..2536787	+	144	WP_042319017.1	stationary-phase-induced ribosome-associated protein	-
HAP28_RS12015	2536959..2538656	+	1698	WP_044257943.1	malate dehydrogenase	-
HAP28_RS12020	2538965..2540680	+	1716	WP_109740239.1	ATP-binding cassette domain-containing protein	-
HAP28_RS12025	2540860..2541138	+	279	WP_044267611.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HAP28_RS12030	2541138..2541422	+	285	WP_043000693.1	HigA family addiction module antidote protein	Antitoxin
HAP28_RS12035	2541467..2542069	-	603	WP_109740240.1	inorganic diphosphatase	-
HAP28_RS12040	2542196..2542852	-	657	WP_043000691.1	formate dehydrogenase-N subunit gamma	-
HAP28_RS12045	2542845..2543729	-	885	WP_043000690.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-83)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10507.00 Da
• Isoelectric Point: 8.1206

>T151990 WP_044267611.1 NZ_CP050009:2540860-2541138 [Citrobacter sp. Y3]
MIINFRHKGLRDLFLHGKTSGVLAKHVKRLRHRLAVIDAAGCVNDINMPGYRLHPLSGDRDGVWSISVSGNWRMTFEFVN
GDAYILDDEDYH

Nucleotide

• Length: 279 bp

>T151990 NZ_CP065867:1239634-1239948 [Pseudomonas aeruginosa]
ATGATCTTCATCGAAACGCCAGTCTTCACGAAGCGGATTCTCGCGCTGGTAGACGACGAGACCTATCGCAAGCTTCAGGA
AGACCTAACGCTCCACCCGGACGCCGGAGTGGTCATTGAAGGCACGGGTGGCGTGCGCAAGATTCGCATTGCTGCCAATG
GCCACGGGAAAAGAGGCGGCGCCCGGGTCATCTACTACCATTTCACATCCGCCTCTCAAATTGCATTCCTGCTGGCCTAT
GACAAAGCATCGCAGGAAGATCTGACTGCCGATCAGAAAAAGATGCTTCGGCAGATCGTTGAGAACTGGAGATAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10370.07 Da
• Isoelectric Point: 10.2982

>AT151990 WP_043000693.1 NZ_CP050009:2541138-2541422 [Citrobacter sp. Y3]
MKMANHPRPGDIIQEALDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPEMWLNLQNTWSLAQAQ
KSVDVSRLRRLAVQ

Nucleotide

• Length: 285 bp

>AT151990 NZ_CP065867:1239951-1240256 [Pseudomonas aeruginosa]
ATGGACAAAGAACTCTTCGCTGAACTGGTCGAAAGCGTGACCCAGATGGACGAGATCGTGCGTGGCGAACGTCAACCTTC
CCGCGAGTTCCACGTCGACGCGGTGAAGGTCAAGGAAATCCGCCGCGCTACTGGCCTGTCCCAGGCCAAATTCGCCCGAA
AGATCGATGTTGCTGTTGGAACCCTGCGCAATTGGGAGCAAGGGCGCCGCGATCCCGAGGGGCCTGCCCGCGCCCTGCTC
CGGGCCATTGACAATGATCCGGTTCATGTTCTGGCTGCCCTGAATGGTGGCCCGCGAGAAAGCTAA

Structures

Toxin

• AlphaFold DB: A0A8B3ZQU4