Detailed information of TA system TA151966

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 735796..736445
• Replicon: chromosome
• Accession: NZ_CP049980
• Organism: Leclercia adecarboxylata strain 707804

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: G7098_RS03400
• Protein ID: WP_165717499.1
• Coordinates: 736083..736445 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G7098_RS03395
• Protein ID: WP_059308573.1
• Coordinates: 735796..736095 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G7098_RS03385	732192..734165	+	1974	WP_165717498.1	PhoX family phosphatase	-
G7098_RS03390	734269..735654	+	1386	WP_077226217.1	phenylalanine transporter	-
G7098_RS03395	735796..736095	-	300	WP_059308573.1	helix-turn-helix transcriptional regulator	Antitoxin
G7098_RS03400	736083..736445	-	363	WP_165717499.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G7098_RS03405	736722..737963	+	1242	WP_077226245.1	MFS transporter	-
G7098_RS03410	737975..739000	+	1026	WP_059308572.1	sugar phosphate isomerase/epimerase	-
G7098_RS03415	738993..740135	+	1143	WP_144883544.1	Gfo/Idh/MocA family oxidoreductase	-
G7098_RS03420	740211..741191	+	981	WP_103791863.1	LacI family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-87)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13984.00 Da
• Isoelectric Point: 7.3565

>T151966 WP_165717499.1 NZ_CP049980:c736445-736083 [Leclercia adecarboxylata]
MWEVETTDTFDKWFNHQTQALKEDVLAIMHILSEYGPQLGRPWVDTVKNSHYANMKELRIQHAGDPVRAFFAFDPVRKAI
ILCAGNKTGKNEKRFYKDMIAMADAEFSDYLASKEAKWLR

Nucleotide

• Length: 363 bp

>T151966 NZ_CP065857:c2095755-2095579 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGTTGGCATTACTGAAATCTTTAGAAAGGAGATGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10972.68 Da
• Isoelectric Point: 5.8526

>AT151966 WP_059308573.1 NZ_CP049980:c736095-735796 [Leclercia adecarboxylata]
MATLKELMAQQSQESQERIAAKVEDLRIIVALNQLREELNISQTELAAAMGVKQPTVAKIEQPDNDPRLSTLKRYVTALG
GEVSIDVILPTGKRVAFHF

Nucleotide

• Length: 300 bp

>AT151966 NZ_CP065857:2095456-2095511 [Staphylococcus aureus]
AAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTG