Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1862566..1863107 Replicon chromosome
Accession NZ_CP049979
Organism Escherichia coli strain IBS28

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag HAP53_RS08735 Protein ID WP_000615976.1
Coordinates 1862566..1862844 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag HAP53_RS08740 Protein ID WP_000729704.1
Coordinates 1862847..1863107 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HAP53_RS08720 1860148..1860726 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
HAP53_RS08725 1860932..1861699 + 768 WP_000333380.1 class II glutamine amidotransferase -
HAP53_RS08730 1861670..1862410 - 741 WP_001225679.1 murein L,D-transpeptidase -
HAP53_RS08735 1862566..1862844 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HAP53_RS08740 1862847..1863107 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HAP53_RS08745 1863293..1864066 + 774 WP_000543895.1 C40 family peptidase -
HAP53_RS08750 1864123..1864479 - 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
HAP53_RS08755 1864472..1864750 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
HAP53_RS08760 1864855..1866567 - 1713 Protein_1693 flagellar biosynthesis protein FlhA -
HAP53_RS08765 1866539..1867324 + 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T151937 WP_000615976.1 NZ_CP049979:c1862844-1862566 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T151937 NZ_CP065841:2034562-2034664 [Klebsiella quasipneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT151937 WP_000729704.1 NZ_CP049979:c1863107-1862847 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT151937 NZ_CP065841:c2034668-2034521 [Klebsiella quasipneumoniae]
AGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTG
GCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGTCTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References