Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1030741..1031540 Replicon chromosome
Accession NZ_CP049979
Organism Escherichia coli strain IBS28

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag HAP53_RS04855 Protein ID WP_000347266.1
Coordinates 1030741..1031205 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HAP53_RS04860 Protein ID WP_001307405.1
Coordinates 1031205..1031540 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HAP53_RS04825 1025742..1026176 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
HAP53_RS04830 1026194..1027072 - 879 WP_155955699.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
HAP53_RS04835 1027062..1027841 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
HAP53_RS04840 1027852..1028325 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HAP53_RS04845 1028348..1029628 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
HAP53_RS04850 1029877..1030686 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HAP53_RS04855 1030741..1031205 - 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HAP53_RS04860 1031205..1031540 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HAP53_RS04865 1031689..1033260 - 1572 WP_001273741.1 galactarate dehydratase -
HAP53_RS04870 1033635..1034969 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
HAP53_RS04875 1034985..1035755 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T151934 WP_000347266.1 NZ_CP049979:c1031205-1030741 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T151934 NZ_CP065841:236326-236631 [Klebsiella quasipneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCTGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT151934 WP_001307405.1 NZ_CP049979:c1031540-1031205 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT151934 NZ_CP065841:236106-236324 [Klebsiella quasipneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGTGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References