Detailed information of TA system TA151932

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 232444..233046
• Replicon: chromosome
• Accession: NZ_CP049979
• Organism: Escherichia coli strain IBS28

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: HAP53_RS01015
• Protein ID: WP_000897305.1
• Coordinates: 232735..233046 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HAP53_RS01010
• Protein ID: WP_000356397.1
• Coordinates: 232444..232734 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HAP53_RS00985	228369..229271	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
HAP53_RS00990	229268..229903	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HAP53_RS00995	229900..230829	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
HAP53_RS01000	231159..231401	-	243	WP_001087409.1	hypothetical protein	-
HAP53_RS01005	231621..231839	-	219	WP_001315930.1	ribbon-helix-helix domain-containing protein	-
HAP53_RS01010	232444..232734	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
HAP53_RS01015	232735..233046	-	312	WP_000897305.1	hypothetical protein	Toxin
HAP53_RS01020	233275..234183	+	909	WP_001162704.1	alpha/beta hydrolase	-
HAP53_RS01025	234247..235188	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HAP53_RS01030	235233..235670	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HAP53_RS01035	235667..236539	-	873	WP_000920762.1	virulence factor BrkB family protein	-
HAP53_RS01040	236533..237132	-	600	WP_001295269.1	glucose-1-phosphatase	-
HAP53_RS01045	237231..238016	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T151932 WP_000897305.1 NZ_CP049979:c233046-232735 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T151932 NZ_CP065840:c1779-1441 [Klebsiella quasipneumoniae]
GTGACGACATATTGTCCAGCCAGAGGCGATGTCATTCTTTTAGATTTTAACCCTCAATCAGGGCATGAGCAGGCAGGGAA
AAGGCCTGCTCTTGTCGTTTCTGATGATTTGTTTAACCAAGTCACTGGGTTTGCTGTTGTTTGCCCCATAACTAATCAGA
TAAAAGGGTACCCTTTTGAAGTTCCTGTTGATGGGACTACTAAAACAACAGGGGTTATCTTGGCTGATCAAGTGAAGTCT
TTAGACTGGAAGGCCAGAGCGGCACGTACTGTTGATAGCGTTTCCGGCGAGACAGTAACTACAGTTGTTGATATGGTTTC
AAAAATTATTAAAGCATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT151932 WP_000356397.1 NZ_CP049979:c232734-232444 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT151932 NZ_CP065840:c2006-1776 [Klebsiella quasipneumoniae]
ATGACTACGGCTATTGGTAAATGGGGTAATGGCGCTGCTTTGCGTATCCCGCAGCCGTTCATGAAGCAGCTTGGGCTTAG
TATCGGTGATGTTGTTAATTTCCGCGTCACTGAAAGGGATTTAGTAATTACTAAAGCTGGCCCGACGCTTGAAGAACTGC
TCGGACAGTGCACTGCAGAAAATCGCCATCACGAGTATCTATGTGACTCTCAAGGTAAGGAGATGCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7