Detailed information of TA system TA151918

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2859983..2860585
• Replicon: chromosome
• Accession: NZ_CP049967
• Organism: Escherichia coli strain ARL09/232

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: G9448_RS14010
• Protein ID: WP_000897305.1
• Coordinates: 2860274..2860585 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G9448_RS14005
• Protein ID: WP_000356397.1
• Coordinates: 2859983..2860273 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G9448_RS13980	2855909..2856811	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
G9448_RS13985	2856808..2857443	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
G9448_RS13990	2857440..2858369	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
G9448_RS13995	2858699..2858941	-	243	WP_001086388.1	hypothetical protein	-
G9448_RS14000	2859160..2859378	-	219	WP_001297075.1	ribbon-helix-helix domain-containing protein	-
G9448_RS14005	2859983..2860273	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
G9448_RS14010	2860274..2860585	-	312	WP_000897305.1	hypothetical protein	Toxin
G9448_RS14015	2860814..2861722	+	909	WP_001162704.1	alpha/beta hydrolase	-
G9448_RS14020	2861786..2862727	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
G9448_RS14025	2862772..2863209	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
G9448_RS14030	2863206..2864078	-	873	WP_000920762.1	virulence factor BrkB family protein	-
G9448_RS14035	2864072..2864671	-	600	WP_001295269.1	glucose-1-phosphatase	-
G9448_RS14040	2864770..2865555	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T151918 WP_000897305.1 NZ_CP049967:c2860585-2860274 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T151918 NZ_CP065834:20126-20608 [Klebsiella pneumoniae]
ATGGGAATGAGAGAGCCTGAATCCCTGACGTCTGAACATAACATCGCTGAGTTCTGCTGCCAGGAGCCTGCCCTTAATGA
GTGGTTAAAGAAGAAGGCTCTCAGAAACCACAGTACCGGCATTTCCCGTGTCTATGTCGTCTGCGCAGAAAACACTAACC
GGGTCATCGGTTATTATTGTTTATCTTCGGGTAGCGTCCACCGTAACACAGTGCCTGGGGCCTATAGACGCAACGCGCCT
GATGTTATTCCAGTCATTGTTTTGGGACGGCTTGCCATCGACCAGGCCTGGTCCGGGAATGGGCTTGGTGCCGCACTTCT
TAAGGATGCTATATACCGTACGCAAAACATTGCCTTTCAGGTCGGGGTGAGAGCACTGGCCGTGCATGCGCTGAATGAAG
AAGTTAAATGTTTTTACACCCGTTTTGGCTTTGAGCCTTCCATAGTCAACACGCTGACATTGTTATTCCCGATAAAAGTC
TGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT151918 WP_000356397.1 NZ_CP049967:c2860273-2859983 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT151918 NZ_CP065834:19857-20135 [Klebsiella pneumoniae]
ATGAAACCAGACGTTCGTGAAGCTCCGATTAATATCCGGGCCAAAACATCACAGCGTGACCTGATCGACATGGCCGCAAG
CCTGGTCTCAAAATCACGGACTGATTTTATGCTTGAGGTTGCCTGCAGGGAGGCACAGGACATTCTGCTTGATCAACGCC
TGTTCCTCCTCAATGACAATCAGTTTGAGGCGTTTATTGAAGAGCTTGACGCCCCAATAACGCCGGAGCGCCAGGCGCGA
ATCGATAACCTGATGAACCGCAAATCACCATGGGAATGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7