Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4714940..4715442 Replicon chromosome
Accession NZ_CP049936
Organism Escherichia coli strain JL05

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag G9V97_RS22250 Protein ID WP_000767829.1
Coordinates 4715188..4715442 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag G9V97_RS22245 Protein ID WP_001259255.1
Coordinates 4714940..4715191 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9V97_RS22220 4710118..4711185 - 1068 WP_109556778.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
G9V97_RS22225 4711185..4712255 - 1071 WP_000108947.1 histidinol-phosphate transaminase -
G9V97_RS22230 4712252..4713556 - 1305 WP_001317939.1 histidinol dehydrogenase -
G9V97_RS22235 4713562..4714461 - 900 WP_000131776.1 ATP phosphoribosyltransferase -
G9V97_RS22240 4714607..4714657 - 51 WP_001364200.1 his operon leader peptide -
G9V97_RS22245 4714940..4715191 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
G9V97_RS22250 4715188..4715442 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
G9V97_RS22255 4715525..4716349 + 825 WP_000754763.1 SDR family oxidoreductase -
G9V97_RS22260 4716395..4717324 + 930 WP_000803351.1 LysR family transcriptional regulator -
G9V97_RS22265 4717539..4717601 + 63 WP_010723108.1 membrane protein YoeI -
G9V97_RS22270 4717591..4718949 + 1359 WP_000019201.1 putrescine/proton symporter PlaP -
G9V97_RS22275 4719128..4720186 + 1059 WP_000492315.1 transport protein YeeE -
G9V97_RS22280 4720200..4720427 + 228 WP_000985280.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T151802 WP_000767829.1 NZ_CP049936:4715188-4715442 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T151802 NZ_CP065743:3254308-3255045 [Bacillus tropicus]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGTAATCGTTTTTCTAGTACTTGTTTTCTTTTATTGGAAAAACGA
AGAGTTGTACGAGGAGAAGAAGCAACGTATTAGAAAGACTTGGTATGGGTTGTTTATAATATCAGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCGAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACAGTTCAATCGATAAAACGTTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATCATTCAAAATGTCAATCCAGCAGTGTTCGGTACGATGG
AATGGCATACGGAGGAAGAATATACAAAAAGCTTAAACGCATTTTTAGATTCGTATGGGGAAAAGATTGGAGCAAAAATT
GTTGTATTTGAAGTGGCAAAGGAATTAAATACAAACTTTCGTGGTATCCGTTCTCAATTTAGTACTATGATTCCGTTAGA
GTACATTGAGCAATTGAATGAGCAGAGAGCAGTACAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAACCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT151802 WP_001259255.1 NZ_CP049936:4714940-4715191 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT151802 NZ_CP065743:3255158-3255283 [Bacillus tropicus]
GTGATAAAAAATAATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACGGATTTCTCGTTAATTAAAGGGGATAC
GATGCAGCAAGTAGAAGATTTAGAAGATGAATTAAAAAGAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References