Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3487515..3488314 Replicon chromosome
Accession NZ_CP049936
Organism Escherichia coli strain JL05

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag G9V97_RS16465 Protein ID WP_000347266.1
Coordinates 3487515..3487979 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag G9V97_RS16470 Protein ID WP_001307405.1
Coordinates 3487979..3488314 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9V97_RS16435 3482516..3482950 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
G9V97_RS16440 3482968..3483846 - 879 WP_001315856.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
G9V97_RS16445 3483836..3484615 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
G9V97_RS16450 3484626..3485099 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
G9V97_RS16455 3485122..3486402 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
G9V97_RS16460 3486651..3487460 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
G9V97_RS16465 3487515..3487979 - 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
G9V97_RS16470 3487979..3488314 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
G9V97_RS16475 3488463..3490034 - 1572 WP_001273741.1 galactarate dehydratase -
G9V97_RS16480 3490409..3491743 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
G9V97_RS16485 3491759..3492529 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3487515..3499075 11560


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T151798 WP_000347266.1 NZ_CP049936:c3487979-3487515 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T151798 NZ_CP065738:c1997783-1997523 [Rothia kristinae]
GTGAAGACGATGACGTATACGGAGTCCCGGGCGCGTTATGCCGAGGTGCTGGACGGCGTGGTGCAGGACCGGGAGGAGGT
GGTGATCACCCGCAGCGGTCACGACCCGGTGGTGATCCTCTCCCTGGCGGAGTATGAGTCGCTGAAGGAGACGGCGCACC
TGCTGCGCTCCCCCTCCAACGCCCGTCGGCTGCTGGATGCGATGCAGCGTTTGGAGGCAGGAGAGGGCGAGCGCCACGAG
CTCGTGGACCCCGATGCCTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT151798 WP_001307405.1 NZ_CP049936:c3488314-3487979 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT151798 NZ_CP065738:c1997530-1997261 [Rothia kristinae]
ATGCCTGACCTGCTGCTGACCTGGGACCCTGACGCCTGGGAGGACTACCTGTGGTGGCAGCGTCAGGATCGCGCCGTGCT
GCGTCGGATCAACACGCTGATCCGGGACATCCAGCGCCACGGGAATGAGGGCATCGGCAAACCGGAGCCGCTTCGTCATG
ACTTCGCCGGATACTGGTCCCGCCGGATCACTGCGGAACACCGACTGGTGTATCGACTCACCGACCGCCAGGTGCTCATC
GCCGCGTGCCGCTATCACTACGGGCAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References