Detailed information of TA system TA151796

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2689141..2689743
• Replicon: chromosome
• Accession: NZ_CP049936
• Organism: Escherichia coli strain JL05

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: G9V97_RS12635
• Protein ID: WP_000897305.1
• Coordinates: 2689432..2689743 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G9V97_RS12630
• Protein ID: WP_000356397.1
• Coordinates: 2689141..2689431 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G9V97_RS12605	2685067..2685969	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
G9V97_RS12610	2685966..2686601	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
G9V97_RS12615	2686598..2687527	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
G9V97_RS12620	2687857..2688099	-	243	WP_001087409.1	hypothetical protein	-
G9V97_RS12625	2688318..2688536	-	219	WP_001315930.1	ribbon-helix-helix domain-containing protein	-
G9V97_RS12630	2689141..2689431	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
G9V97_RS12635	2689432..2689743	-	312	WP_000897305.1	hypothetical protein	Toxin
G9V97_RS12640	2689972..2690880	+	909	WP_001162704.1	alpha/beta hydrolase	-
G9V97_RS12645	2690944..2691885	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
G9V97_RS12650	2691930..2692367	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
G9V97_RS12655	2692364..2693236	-	873	WP_000920762.1	virulence factor BrkB family protein	-
G9V97_RS12660	2693230..2693829	-	600	WP_001295269.1	glucose-1-phosphatase	-
G9V97_RS12665	2693928..2694712	-	785	Protein_2469	DeoR/GlpR transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T151796 WP_000897305.1 NZ_CP049936:c2689743-2689432 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T151796 NZ_CP065729:c1494558-1494208 [Macrococcus caseolyticus]
ATGAAACGCGGAGATGTTTATTTAGCTGATCTGTCACCAGTGAAGGGGTCTGAACAAGGCGGTAAACGCCCCGTCGTTAT
TATTCAGAATAATATTGGGAATAAACATAGTCCTACAGTGATTGTAGCAGCAATTACTGCAAAGATTAATAAGGCGAAAA
TCCCCACCCATGTTGAAATTGGCAAGACAACGCATCGCTTAGAAAAGGATTCGGTAATACTGCTGGAACAAATTAGAACC
ATTGATAAGAATAGGTTGATTGATCATTTAACTGTGCTTGATAAGGCGAAGATGAAAGAAGTGGATACTGCACTTATAAT
TAGTTTGGGTTTATCAATCGATAGTGAATAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT151796 WP_000356397.1 NZ_CP049936:c2689431-2689141 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT151796 NZ_CP065729:c1494699-1494559 [Macrococcus caseolyticus]
ATGAGCCAGAAGCTAGAACAAATGCTGATAGAAGGTTATCAATCAATGGCAGAGATAAATTTATCGATAGCATCAGAACA
TTATGTATTAGAATGTGAAGTTACTGATAACTATCAGACATATACCTTTGATGGTGAATAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7