Detailed information of TA system TA151766

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 1644592..1645205
• Replicon: chromosome
• Accession: NZ_CP049918
• Organism: Bordetella bronchiseptica strain KVNON-570

Toxin (Protein)

• Gene name: higB
• Uniprot ID: K0MEM3
• Locus tag: G6545_RS07610
• Protein ID: WP_003811545.1
• Coordinates: 1644894..1645205 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G6545_RS07605
• Protein ID: WP_003811547.1
• Coordinates: 1644592..1644891 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6545_RS07595	1640700..1641374	-	675	WP_003811550.1	response regulator transcription factor	-
G6545_RS07600	1641504..1644578	+	3075	WP_165645803.1	autotransporter SphB2	-
G6545_RS07605	1644592..1644891	-	300	WP_003811547.1	helix-turn-helix domain-containing protein	Antitoxin
G6545_RS07610	1644894..1645205	-	312	WP_003811545.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G6545_RS07615	1645319..1645942	-	624	WP_003811542.1	glutathione S-transferase family protein	-
G6545_RS07620	1646186..1647055	+	870	WP_010930493.1	TauD/TfdA family dioxygenase	-
G6545_RS07625	1647033..1647977	-	945	WP_080702971.1	LysR family transcriptional regulator	-
G6545_RS07630	1648063..1648989	-	927	WP_033461661.1	tripartite tricarboxylate transporter substrate binding protein	-
G6545_RS07635	1649102..1649881	-	780	WP_165645804.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (42-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-96)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11810.72 Da
• Isoelectric Point: 8.8245

>T151766 WP_003811545.1 NZ_CP049918:c1645205-1644894 [Bordetella bronchiseptica]
MEFIETPIFTQLITALLSDEEYSRLQQLLIDNPERGDLLRGGGGIRKMRYGRQGTGKRGGIRVIYYWISQDCQIYMLAAY
AKSKKINLTPDEIAALRELVKEL

Nucleotide

• Length: 312 bp

>T151766 NZ_CP065700:c4063983-4063615 [Atlantibacter hermannii]
ATGACAATTCAGTTTATCTCAGCGGAAGAGATAATTCGTTTTCATGACAGATTGCTGGCCGTCACGCCAGGCGTACCCGG
TATGGTCGATCCGGGTCGGGCGGAAGCACTGTTATACCGTGTGCTTAACAAATACAAGTATGAAGGCGTTAATGACCTCT
GGCTTTTGGCAGCAATGCACCTGCTGGCTATTTCCCGAGGGCATATCTTCAATGATGGCAATAAACGCACGGCGCTGTTT
ATTACCTTGTTGTTCCTTAAGCGCAATGGGATAACGCTTCCCGCCAACCCGGCATTTGTCGAGTTAACGGTGGCGGCGGC
GGCAGGACAACTTAGCCTTGAAGAGATAGCGCGTCAGTTACGAAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10946.70 Da
• Isoelectric Point: 11.2467

>AT151766 WP_003811547.1 NZ_CP049918:c1644891-1644592 [Bordetella bronchiseptica]
MKKDNTVFNDLQASLKQAVAIRQGKMPPSRVFKVETVDVKATREKTHMSQTEFAKAIHVSVRTLQNWEQGHRNPTGPAVA
LLKIVASAPDIAVRALRTA

Nucleotide

• Length: 300 bp

>AT151766 NZ_CP065700:c4064201-4063980 [Atlantibacter hermannii]
ATGCAAACCATGAACTATAGCGAAGCGCGCCAGAATCTTGCTGCCGCGCTCGAGACAGCTGCGCTGGGTACGCCTGTTAC
TATCACCCGTCGGGGCCACAAACCTGCGGTAATTATTAGCGCCGAAGAATTCGAACGTTATCAACAAGCAAAGATGGATG
CCGAATTTGCCGCCATCATGGCTATTCATGGCAATGAAATCAGGGAACTTGCCGATAAATGA

Structures

Toxin

• AlphaFold DB: K0MEM3