Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2842186..2842688 Replicon chromosome
Accession NZ_CP049852
Organism Escherichia coli strain UPEC-774U/190308

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag G9W34_RS14050 Protein ID WP_000767822.1
Coordinates 2842186..2842440 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag G9W34_RS14055 Protein ID WP_001259255.1
Coordinates 2842437..2842688 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9W34_RS14025 (2837188) 2837188..2838615 + 1428 WP_001538915.1 exodeoxyribonuclease I -
G9W34_RS14030 (2838689) 2838689..2840047 - 1359 WP_000019194.1 putrescine/proton symporter PlaP -
G9W34_RS14035 (2840037) 2840037..2840099 - 63 WP_010723108.1 membrane protein YoeI -
G9W34_RS14040 (2840314) 2840314..2841233 - 920 Protein_2756 LysR substrate-binding domain-containing protein -
G9W34_RS14045 (2841279) 2841279..2842103 - 825 WP_000754737.1 SDR family oxidoreductase -
G9W34_RS14050 (2842186) 2842186..2842440 - 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
G9W34_RS14055 (2842437) 2842437..2842688 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
G9W34_RS14060 (2842971) 2842971..2843021 + 51 WP_001364200.1 his operon leader peptide -
G9W34_RS14065 (2843167) 2843167..2844066 + 900 WP_000131779.1 ATP phosphoribosyltransferase -
G9W34_RS14070 (2844072) 2844072..2845376 + 1305 WP_001445483.1 histidinol dehydrogenase -
G9W34_RS14075 (2845373) 2845373..2846443 + 1071 WP_001538918.1 histidinol-phosphate transaminase -
G9W34_RS14080 (2846443) 2846443..2847510 + 1068 WP_001538920.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T151728 WP_000767822.1 NZ_CP049852:c2842440-2842186 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T151728 NZ_CP065688:2769722-2770144 [Serratia plymuthica]
GTGGCCCAGTGGCGATGTAATGTCCGCATTTCCTGGCGCACCCAGTTACTTTCGCTGCTGACCCACGGCGTTCTGATCCT
GCTGATCCTGATTTCGCCCTGGCCGGAAGGCTACGGCCCGATCTGGCTGGTGTTGTTGACGCTGGTGGTATTTGAATGCA
TCCGCAGCCAAAAGCGCATTGCGTCGCGGCAGGGCGAACTGCGTCTGCTGGCGAATAAGCGGTTAAGTTGGCACGGACAG
GAATGGTTGCTGGCAAAGCAACCCTGGATGCCGCGTTACGGCATTTTGCTGACGCTGCAGCCGACGCAGGGCAAAAAACG
CCGCCGGCTGTGGCTGGCCTCCGACAGCATGGGCAAAGCCGAATGGCGGCATCTGCGCCAGTTGCTGCTGCACCCGTCGG
CCGATGATGACGAGGAACCCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT151728 WP_001259255.1 NZ_CP049852:c2842688-2842437 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT151728 NZ_CP065688:2769475-2769741 [Serratia plymuthica]
ATGGATATTAATAATAAAGCACGTATTCACTGGGCCTGCCGCCGTGGCATGCGTGAGCTGGACATCTCAATCATGCCGTT
CTTCGAGCATGAGTACGACCAGTTGAATGACACAGACAAAGCGTTGTTTATCCGCCTGCTTGAGAGCGACGATCCCGATC
TGTTCAACTGGTTGATGAATCATGGCGCTCCGCAAGACAGTGAACTGCAGAGAATGGTGACACTGATCCAGACGCGAAAT
AAAGACCGTGGCCCAGTGGCGATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References